Genetically Transformed Strawberry (Fragaria x ananassa Duch.) with Cold-Inducible Transcription Factor CBF1
文献类型： 外文期刊
第一作者： Jin, W. M.
作者： Jin, W. M.;Dong, J.;Liu, Y.;Zhang, Y. P.;Pan, Q. H.
作者机构：
关键词： strawberry;gene CBF1;genetic transformation;cold-resistance
期刊名称：VI INTERNATIONAL STRAWBERRY SYMPOSIUM
ISSN： 0567-7572
年卷期： 2009 年 842 卷
页码：
收录情况： SCI
摘要： A foreign target-gene of cold-inducible transcription factor CBF1 (C-repeat binding factor) from Arabidopsis thaliana was introduced into strawberry by using the leaf-disc method via Agrobacterium tumefaciens. The transgenic plants were subjected to screening by Kan 25 mg/L, and then polymerase chain reaction (PCR) and Southern hybridization. The analysis of results indicated that the CBF1 gene integrated into the genome of strawberry. An analysis of physiological characteristics in terms of cold resistance was undertaken, based on methods of electrolyte leakage and restored growth. The results indicated that electrical conductivity of transgenic plants was lower than that of the control plant 'Toyonoka'. All transgenic plants and 'Toyonoka' were put in a temperature of -2 degrees C for 7 days and 60% of transgenic plants, whereas 85% of control plants wilted. Afterwards they were put in a temperature of 22 to 25 degrees C for restored growth. There, 44% of wilted transgenic plants and 30% of control plants were recovered. The introduction of CBF1 to strawberry may strengthen its resistance to the cold condition.
分类号：
- 相关文献
作者其他论文 更多>>
-
Supplementing pigeon grit with acidifier improves metabolism and the reproductive performance of breeding pigeons as well as the development of growth performance of squabs
作者：Zheng, S.;Tang, Y.;Huang, W.;Zhang, W.;Zhang, Y.;Yang, M.;Lu, H.;Li, Z.;He, Y.;Gao, H.;Wang, W.;Peng, J.;Huang, Y.;Liang, Y.;Qiu, X.;Liu, Y.;Gou, Z.;Qiu, Z.;Bin, Y.;Zhang, Z.;Wang, W.;Huang, Y.
关键词：Acidifier; breeder pigeon; squab; performance; metabolism
-
Organic selenium supplementation increases serum selenium levels in healthy Xinjiang brown cattle fed selenised yeast
作者：Li, Z.;Tang, J.;Li, J.;Ling, D.;He, X.;Tang, Y.;Yi, P.;Yang, Y.;Li, Z.;Li, J.;He, X.;Tang, Y.;Yi, P.;Tang, J.;Khoo, H. E.;Liu, Y.
关键词：blood metabolites; Bos taurus; enzyme activity; red dates; slaughter performance
-
Comparative transcriptomic analyses reveal key genes underlying melanin distribution during embryonic development in geese (Anser anser)
作者：Yang, Y.;Wang, C.;Liu, Y.;Li, G.;Wang, X.;Wang, H.;He, D.
关键词：Graylag geese; melanin; genetics; dorsal skin; distal feet
-
Effects of fermented cottonseed meal inclusions on growth performance, serum biochemical parameters and hepatic lipid metabolism of geese during 28-70 d of age
作者：Xie, S. S.;Shen, J. J.;Liu, Y.;Wang, W. C.;Yang, L.;Zhu, Y. W.;Yang, Z. L.
关键词：goose; fermented cottonseed meal; growth performance; serum biochemical parameters; hepatic lipid metabolism
-
Transcriptome analysis reveals heat shock protein 70 (HSP70) induced by tembusu virus infection is associated with immune responses
作者：Zhao, D.;Han, K.;Zhang, L.;Huang, X.;Liu, Q.;Yang, J.;Liu, Y.;Li, Y.;Wu, F.;Zhao, D.;Zhao, D.;Zhao, D.;Han, K.;Zhang, L.;Huang, X.;Liu, Q.;Yang, J.;Liu, Y.;Li, Y.;Wu, F.
关键词：heat shock protein 70; innate immune response; tembusu virus; transcriptome analysis
-
Nutrient profiles and browning control of wasp larvae
作者：Wang, K.;Zhu, H.;Chen, X.;Qiao, J.;Dong, J.;Zhang, H.;Wang, K.;Qiao, J.;Haubruge, E.;Huang, G.;Dong, J.;Zhang, H.
关键词：ascorbic acid treatment; edible insects; high hydrostatic pressure; phenol oxidase; umami
-
Low-depth Raw Reads of Nanopore Sequencing Enables Rapid and Accurate Bacterial Identification
作者：Gao, W.;Hong, W.;Wu, Y.;Liu, Y.;Fan, X.;Wang, H.;Wang, M.;Yang, B.;Wang, T.;Li, H.;Liu, S.;Li, J.
关键词：assembly; reads; Nanopore sequencing; Illumina sequencing; bacterial identification
