Molecular cloning, identification, and chromosomal localization of two MADS box genes in peach (Prunus persica)

文献类型： 外文期刊

第一作者： Zhang, Lin

作者： Zhang, Lin;Ma, Rongcai;Zhang, Lin;Xu, Yong;Ma, Rongcai

作者机构：

关键词： peach (Prunus persica);flower development;MADS box gene;selective mapping

期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：4.275； 五年影响因子：5.223 ）

ISSN： 1673-8527

年卷期： 2008 年 35 卷 6 期

页码：

收录情况： SCI

摘要： MADS box proteins play an important role in floral development. To find genes involved in the floral transition of Prunus species, cDNAs for two MADS box genes, PpMADS1 and PpMADS10, were cloned using degenerate primers and 5'- and 3'-RACE based on the sequence database of P. persica and P. dulcis. The full length of PpMADS1 cDNA is 1,071 by containing an open reading frame (ORF) of 717 by and coding for a polypeptide of 238 amino acid residues. The full length of PpMADS10 cDNA is 937 by containing an ORF of 633 by and coding for a polypeptide of 210 amino acid residues. Sequence comparison revealed that PpMADS1 and PpMADS10 were highly homologous to genes AP1 and PI in Arabidopsis, respectively. Phylogenetic analysis indicated that PpMADS1 belongs to the euAP1 clade of class A, and PpMADS10 is a member of GLO/PI clade of class B. RT-PCR analysis showed that PpMADS1 was expressed in sepal, petal, carpel, and fruit, which was slightly different from the expression pattern of AP1; PpMADS10 was expressed in petal and stamen, which shared the same expression pattern as PI. Using selective mapping strategy, PpMADS1 was assigned onto the Bin1:50 on the G1 linkage group between the markers MCO44 and TSA2, and PpMADS10 onto the Bin1:73 on the same linkage group between the markers Lap-1 and FGA8. Our results provided the basis for further dissection of the two MADS box gene function.

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