Laser Irradiation-Induced DNA Methylation Changes Are Heritable and Accompanied with Transpositional Activation of mPing in Rice

文献类型： 外文期刊

第一作者： Li, Siyuan

作者： Li, Siyuan;Xia, Qiong;Yu, Xiaoming;Gao, Xiang;Liu, Bao;Li, Siyuan;Wang, Fang;Ma, Jian;Kou, Hongping;Lin, Xiuyun

作者机构：

关键词： Nd(3+)YAG laser irradiation;DNA methylation;transposable elements;epigenetic inheritance;rice

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： DNA methylation is an integral component of the epigenetic code in most higher eukaryotes. Exploring the extent to which DNA methylation can be altered under a specific condition and its heritability is important for elucidating the biological functions of this epigenetic modification. Here, we conducted MSAP analysis of rice plants with altered phenotypes subsequent to a low-dose Nd(3+)YAG laser irradiation. We found that all four methylation patterns at the 5'-CCGG sites that are analyzable by MSAP showed substantial changes in the immediately treated MO plants. Interestingly, the frequencies of hypo- and hypermethylation were of similar extents, which largely offset each other and render the total methylation levels unchanged. Further analysis revealed that the altered methylation patterns were meiotically heritable to at least the M2 generation but accompanied with further changes in each generation. The methylation changes and their heritability of the metastable epigenetic state were verified by bisulfite sequencing of portion of the retrotranspon, Tosl 7, an established locus for assessing DNA methylation liability in rice. Real-time PCR assay indicated that the expression of various methylation-related chromatin genes was perturbed, and a Pearson correlation analysis showed that many of these genes, especially two AGOs (AG04-1 and AGO4-2), were significantly correlated with the methylation pattern alterations. In addition, excisions of a MITE transposon, mPing, occurred rampantly in the laser irradiated plants and their progenies. Together, our results indicate that heritable DNA methylation changes can be readily induced by low-dose laser irradiation, and which can be accompanied by transpostional activation of transposable elements.

分类号：

  • 相关文献

[1]Heritable alteration in DNA methylation pattern occurred specifically at mobile elements in rice plants following hydrostatic pressurization. Long, LK,Lin, XY,Zhai, JZ,Kou, HP,Yang, W,Liu, B. 2006

[2]Functional analysis of GUS expression patterns and T-DNA integration characteristics in rice enhancer trap lines. Peng, H,Huang, HM,Yang, YZ,Zhai, Y,Wu, JX,Huang, DF,Lu, TG.

[3]Comparative analysis of DNA methylation changes in two rice genotypes under salt stress and subsequent recovery. Wang, Wensheng,Huang, Fei,Qin, Qiao,Zhao, Xiuqin,Li, Zhikang,Fu, Binying,Li, Zhikang,Fu, Binying.

[4]Genome-Wide Differences in DNA Methylation Changes in Two Contrasting Rice Genotypes in Response to Drought Conditions. Wang, Wensheng,Qin, Qiao,Sun, Fan,Wang, Yinxiao,Xu, Dandan,Li, Zhikang,Fu, Binying,Xu, Dandan,Li, Zhikang,Fu, Binying. 2016

[5]DNA methylation changes detected by methylation-sensitive amplified polymorphism in two contrasting rice genotypes under salt stress. Wang, Wensheng,Zhao, Xiuqin,Pan, Yajiao,Zhu, Linghua,Fu, Binying,Li, Zhikang,Wang, Wensheng,Li, Zhikang. 2011

[6]DNA methylation alterations of rice in response to cold stress. Pan, Yajiao,Wang, Wensheng,Zhao, Xiuqin,Zhu, Linghua,Fu, Binying,Li, Zhikang. 2011

[7]DNA Methylation Changes Induced in Rice by Exposure to High Concentrations of the Nitric Oxide Modulator, Sodium Nitroprusside. Ou, Xiufang,Zhuang, Tingting,Yin, Wenchao,Miao, Yiling,Wang, Bo,Zhang, Yunhong,Lin, Xiuyun,Xu, Chunming,Liu, Bao,Zhuang, Tingting,Lin, Xiuyun,von Wettstein, Diter,Rustgi, Sachin,von Wettstein, Diter,Rustgi, Sachin.

[8]Heritable alteration in DNA methylation induced by nitrogen-deficiency stress accompanies enhanced tolerance by progenies to the stress in rice (Oryza sativa L.). Von Wettstein, D.,Kou, H. P.,Li, Y.,Song, X. X.,Ou, X. F.,Liu, B.,Kou, H. P.,Li, Y.,Song, X. X.,Ou, X. F.,Liu, B.,Kou, H. P.,Ma, J.,Xing, S. C.. 2011

[9]Rapid alterations of gene expression and cytosine methylation in newly synthesized Brassica napus allopolyploids. Xu, Yanhao,Zhong, Lan,Wang, Jianbo,Wu, Xiaoming,Fang, Xiaoping.

[10]Megabase Level Sequencing Reveals Contrasted Organization and Evolution Patterns of the Wheat Gene and Transposable Element Spaces. Choulet, Frederic,Rustenholz, Camille,Paux, Etienne,Salse, Jerome,Leroy, Philippe,Feuillet, Catherine,Wicker, Thomas,Keller, Beat,Schlub, Stephane,Le Paslier, Marie-Christine,Brunel, Dominique,Magdelenat, Ghislaine,Gonthier, Catherine,Couloux, Arnaud,Budak, Hikmet,Breen, James,Appels, Rudi,Pumphrey, Michael,Gill, Bikram S.,Liu, Sixin,Anderson, James A.,Kong, Xiuying,Jia, Jizeng,Gut, Marta.

[11]SDG714, a histone H3K9 methyltransferase, is involved in Tos17 DNA methylation and transposition in rice. Ding, Yong,Wang, Xia,Su, Lei,Zhai, JiXian,Cao, ShouYun,Zhang, DongFen,Liu, ChunYan,Bi, YuPing,Qian, Qian,Cheng, ZhuKuan,Chu, ChengCai,Cao, XiaoFeng.

[12]Shifts in the evolutionary rate and intensity of purifying selection between two Brassica genomes revealed by analyses of orthologous transposons and relics of a whole genome triplication. Zhao, Meixia,Tong, Chaobo,Yu, Jingyin,Huang, Shunmou,Liu, Shengyi,Zhao, Meixia,Du, Jianchang,Lin, Feng,Ma, Jianxin,Wang, Xiaowu. 2013

[13]High-resolution genetic mapping of maize pan-genome sequence anchors. Lu, Fei,Romay, Maria C.,Glaubitz, Jeffrey C.,Elshire, Robert J.,Buckler, Edward S.,Bradbury, Peter J.,Buckler, Edward S.,Wang, Tianyu,Li, Yu,Li, Yongxiang,Semagn, Kassa,Zhang, Xuecai,Hernandez, Alvaro G.,Mikel, Mark A.,Mikel, Mark A.,Soifer, Ilya,Barad, Omer.

[14]Mobilization of Diverse Transposable Elements in Rice Induced by Alien Pollination Without Entailing Genetic Introgression. Wu, Ying,Guo, Guizhen,Lin, Xiuyun,Wu, Ying,Jiang, Tingting,Sun, Yue,Wang, Zeyang,Sun, Shuai,Li, Ning,Wang, Zhenhui,Zhang, Di,Bai, Yan,Gao, Yang,Liu, Bao,Dong, Yuzhu.

[15]Widespread and evolutionary analysis of a MITE family Monkey King in Brassicaceae. Dai, Shutao,Hou, Jinna,Long, Yan,Wang, Jing,Li, Cong,Xiao, Qinqin,Jiang, Xiaoxue,Zou, Xiaoxiao,Zou, Jun,Meng, Jinling,Hou, Jinna. 2015

[16]Genome-wide high-resolution mapping of DNA methylation identifies epigenetic variation across embryo and endosperm in Maize (Zea may). Wang, Pengfei,Ma, Chuanxi,Wang, Pengfei,Xia, Han,Zhang, Ye,Zhao, Shuzhen,Zhao, Chuanzhi,Hou, Lei,Li, Changsheng,Li, Aiqin,Wang, Xingjun. 2015

[17]Synonymous Codon Usage Bias in the Plastid Genome is Unrelated to Gene Structure and Shows Evolutionary Heterogeneity. Qi, Yueying,Xu, Wenjing,Xing, Tian,Zhao, Mingming,Yan, Li,Xia, Guangmin,Wang, Mengcheng,Li, Nana. 2015

[18]Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos. Huan, Yan Jun,Zhu, Jiang,Xie, Bing Teng,Wang, Jian Yu,Li, Jing Yu,Xue, Bing Hua,Kong, Qing Ran,Liu, Zhong Hua,Huan, Yan Jun,Wu, Zhan Feng,Zhang, Ji Guang.

[19]Study on DNA Cytosine Methylation of Cotton (Gossypium hirsutum L.) Genome and Its Implication for Salt Tolerance. ZHAO Yun-lei,YU Shu-xun,YE Wu-wei,WANG Hong-mei,WANG Jun-juan,FANG Bao-xing. 2010

[20]Induced Pib Expression and Resistance to Magnaporthe grisea are Compromised by Cytosine Demethylation at Critical Promoter Regions in Rice. Li, Yuan,Xia, Qiong,Wu, Ying,Xu, Chunming,Liu, Bao,Li, Yuan,Xia, Qiong,Wu, Ying,Xu, Chunming,Liu, Bao,Kou, Hongping,Wang, Dan,Lin, Xiuyun,Xing, Shaochen. 2011

作者其他论文 更多>>

微信小程序