Genome shuffling of Lactobacillus plantarum C88 improves adhesion
文献类型: 外文期刊
第一作者: Zhao, Yujuan
作者: Zhao, Yujuan;Duan, Cuicui;Gao, Lei;Yu, Xue;Niu, Chunhua;Li, Shengyu
作者机构:
关键词: probiotics;mutagenesis;lactobacilli;genome shufFLing;adhesion
期刊名称:BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY ( 影响因子:2.043; 五年影响因子:2.017 )
ISSN: 0916-8451
年卷期: 2017 年 81 卷 1 期
页码:
收录情况: SCI
摘要: Genome shuffling is an important method for rapid improvement in microbial strains for desired phenotypes. In this study, ultraviolet irradiation and nitrosoguanidine were used as mutagens to enhance the adhesion of the wild-type Lactobacillus plantarum C88. Four strains with better property were screened after mutagenesis to develop a library of parent strains for three rounds of genome shuffling. Fusants F3-1, F3-2, F3-3, and F3-4 were screened as the improved strains. The in vivo and in vitro tests results indicated that the population after three rounds of genome shuffling exhibited improved adhesive property. Random Amplified Polymorphic DNA results showed significant differences between the parent strain and recombinant strains at DNA level. These results suggest that the adhesive property of L. plantarum C88 can be significantly improved by genome shuffling. Improvement in the adhesive property of bacterial cells by genome shuffling enhances the colonization of probiotic strains which further benefits to exist probiotic function.
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