Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco

文献类型： 外文期刊

第一作者： Wang, Yun-Peng

作者： Wang, Yun-Peng;Wei, Zheng-Yi;Zhong, Xiao-Fang;Lin, Chun-Jing;Zhang, Yu-Ying;Liu, Yan-Zhi;Xing, Shao-Chen;Cai, Yu-Hong;Ma, Jian;Zhang, Yu-Ying

作者机构：

关键词： tobacco;chloroplast;genetic transformation;basic fibroblast growth factor;green fluorescent protein

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2016 年 17 卷 1 期

页码：

收录情况： SCI

摘要： Basic fibroblast growth factor (bFGF) is a multifunctional factor in acceleration of cell proliferation, differentiation and transference, and therefore widely used in clinical applications. In this study, expression vector pWX-Nt03 harboring a codon-optimized bFGF gene was constructed and introduced into the tobacco chloroplasts by particle bombardment. After four rounds of selection, bFGF was proved to integrate into the chloroplast genome of regenerated plants and two of four transgenic plants were confirmed to be homoplastomic by PCR and Southern hybridization. ELISA assay indicated that bFGF represented approximately 0.1% of total soluble protein in the leaves of transplastomic tobacco plants. This is the first report of bFGF expression via chloroplast transformation in model plant, providing an additional option for the production of chloroplast-produced therapeutic proteins.

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