A screening method for detecting simple sequence repeat (SSR) polymorphism of Zea mays using high-resolution melting-curve analysis
文献类型: 外文期刊
第一作者: Jiang, Y.
作者: Jiang, Y.;Tan, H.;Li, Y. D.;Yu, R. H.;Wang, S.;Li, X. H.;Shan, X. H.
作者机构:
关键词: High-resolution melting-curve;SSR;Zea mays
期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )
ISSN: 1684-5315
年卷期: 2011 年 10 卷 73 期
页码:
收录情况: SCI
摘要: As a very important molecular marker technique, simple sequence repeat (SSR) is widely used in marker assistant selection and maize DNA fingerprint. High-resolution melting-curve (HRM) analysis is a new technique to detect the sequence differences in polymerase chain reaction (PCR) amplicons, which is quicker, more accurate and effective than electrophoresis analysis. In this study, we developed a HRM system to detect SSR polymorphism of Zea mays. We successfully used this method to identify genotypes of F2 generation and detecting the SSR polymorphism in different maize inbred lines, therefore, this HRM-SSR system can be used to substitute the electrophoresis after SSR PCR amplification.
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