Changes in H2O2 content and antioxidant enzyme gene expression during the somatic embryogenesis of Larix leptolepis

文献类型： 外文期刊

第一作者： Zhang, Shou-gong

作者： Zhang, Shou-gong;Yang, Wen-hua;Wei, Hua-li;Qi, Li-wang;Han, Su-ying;Zhang, Ming

作者机构：

关键词： Somatic embryogenesis;Hydrogen peroxide;Catalase;Superoxide dismutase;Ascorbate peroxidase;L. leptolepis

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN： 0167-6857

年卷期： 2010 年 100 卷 1 期

页码：

收录情况： SCI

摘要： Hydrogen peroxide (H2O2) content and transcript levels of genes encoding superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) antioxidant enzymes were investigated during different stages of somatic embryogenesis in Larix leptolepis. H2O2 content was lowest on day 0 when embryogenic callus was incubated on Murashige and Skoog (MS) medium supplemented with polyethylene glycol (PEG). This content began to increase when callus was transferred to the same medium but containing abscisic acid (ABA), and reached a peak at day 3 following incubation. The level of H2O2 dropped from day 7 to day 10, peaked at day 21, then dropped again at day 24 and day 35, but increased when somatic embryos reached maturity at day 45. Transcript levels of SOD, CAT, and APX were lowest when somatic embryos were cultured on callus induction medium without ABA. When calli were transferred onto somatic embryo maturation medium, expression patterns of SOD, CAT, and APX varied, and transcript levels at all stages were higher than those at d 0. SOD expression was highest at day 3. Whereas, CAT expression levels were low during early stages of somatic embryogenesis, but increased at day 21, declined at days 24 and 35, and then began to increase again at day 45. APX gene expression patterns were highest at days 3, 21, and 45. These results suggested that ABA was essential for promoting somatic embryogenesis of L. leptolepis. Moreover, ABA induced production of H2O2 and other active oxygen species (AOS), and mediated CAT, SOD, and APX gene expression in somatic embryogenesis of L. leptolepis.

分类号：

  • 相关文献

[1]Comparison of jasmine antioxidant system responses to different degrees and durations of shade. Deng, Yanming,Jia, Xinping,Sun, Xiaobo,Liang, Lijian,Su, Jiale. 2018

[2]Effects of excess copper on the oxidative stress in roots of maize seedlings. Wang, Yan Zhao,Nie, Li Hong,Tie, Shuanggui,Xie, Deyi,Zhu, Weihong,Qi, Jianshuang,Yue, Runqing. 2011

[3]Improved tolerance toward low temperature in banana (Musa AAA Group Cavendish Williams). Chen, Y. J.,Sun, L. L.,Song, L. Y.,Peng, C. L.,Zhang, J. Z.,Zhang, J. Z.. 2012

[4]Protection of photosynthesis and antioxidative system by 24-epibrassinolide in Solanum melongena under cold stress. Wu, X. X.,Zha, D. S.,Wu, X. X.,Zhu, Z. W.,Yang, S. J.,Zha, D. S.,He, J.. 2014

[5]Amelioration of salt-induced oxidative stress in eggplant by application of 24-epibrassinolide. Ding, H-D,Zhu, Z-W,Yang, S-J,Zha, D-S,Wu, X-X,Ding, H-D,Zhu, X-H,Wu, X-X.

[6]Enhanced preservation effects of sugar apple fruits by salicylic acid treatment during post-harvest storage. Mo, Yiwei,Gong, Deqiang,Xie, Jianghui,Li, Weicai,Liang, Guobin,Han, Ruihong.

[7]Response of xanthophyll cycle and chloroplastic antioxidant enzymes to chilling stress in tomato over-expressing glycerol-3-phosphate acyltransferase gene. Sui, N.,Li, M.,Liu, X.-Y.,Wang, N.,Fang, W.,Meng, Q. -W.. 2007

[8]Effects on liver hydrogen peroxide metabolism induced by dietary selenium deficiency or excess in chickens. Xu, Jing-Xiu,Cao, Chang-Yu,Wang, Li-Li,Li, Nan,Xu, Shi-Wen,Li, Jin-Long,Sun, Yan-Chun.

[9]Exogenous 24-epibrassinolide ameliorates high temperature-induced inhibition of growth and photosynthesis in Cucumis melo. Zhang, Y. P.,Chen, Y. Y.,Zhang, Y. P.,Yang, S. J.,Chen, Y. Y.,He, J..

[10]Zinc improves salt tolerance by increasing reactive oxygen species scavenging and reducing Na+ accumulation in wheat seedlings. Xu, L. H.,Wang, W. Y.,Xu, J.,Xu, L. H.,Guo, J. J.,Shi, D. Q.,Xu, J.,Qin, J.,Shi, D. Q.,Li, Y. L..

[11]Enhanced alkaline catalase production by Serratia marcescens FZSF01: Enzyme purification, characterization, and recombinant expression. Jia, Xianbo,Lin, Xinjian,Lin, Chenqiang,Chen, Jichen,Jia, Xianbo,Lin, Lirong,Chen, Jichen. 2017

[12]OxyR-regulated catalase CatB promotes the virulence in rice via detoxifying hydrogen peroxide in Xanthomonas oryzae pv. oryzae. Wang, Nu,Wu, Maosen,Tian, Fang,Chen, Huamin,Yang, Fenghuan,He, Chenyang,Yuan, Xiaochen,Yang, Ching-Hong. 2016

[13]Growth rate, catalase and superoxide dismutase activities in rock carp (Procypris rabaudi Tchang) exposed to supersaturated total dissolved gas. Li, Ke-feng,Li, Jia,Li, Ran,Du, Jun. 2011

[14]Effects of P Deficiency on Protective Enzyme Activity and Membrane Lipid Peroxidation in Different Tomato Genotypes. Wan, Yanhui,Zhu, Longying,Yang, Shaojun,Yang, Zhijie,Zhu, Weimin. 2010

[15]Effects of Soybean Isoflavones on In vitro Antioxidative Capacity of Satellite Cells of Porcine Skeletal Muscles. Jiang Zong-yong,Zhou Gui-lian,Lin Ying-cai,Jiang Shou-qun,Zheng Chun-tian,Han Li-ming,Chen Fang. 2011

[16]Effect of iron supplementation on intestinal function and oxidative stress in piglets with induced colitis. Chen, Q.,Le, G. W.,Shi, Y. H.,Zhang, Sh. M.,Jin, X..

[17]Microcystin-RR-induced accumulation of reactive oxygen species and alteration of antioxidant systems in tobacco BY-2 cells. Yin, LY,Huang, JQ,Huang, WM,Li, DH,Wang, GH,Liu, YD.

[18]The impact of co-infection by a nucleopolyhedrovirus and the endoparasitoid Microplitis pallidipes Szepligeti on the protective enzymes in hemolymph of Spodoptera exigua. Wan, Nian-Feng,Ji, Xiang-Yun,Yin, Yang-Yi,Zhang, Hao,Jiang, Jie-Xian.

[19]Investigations into the Effects of Microcystin-LR on the Growth and Antioxidant Enzymes in Chinese cabbage and Rape. Xue Yanfeng, I,Li Youqin, II,Shi Zhiqi,Yi Neng, III. 2010

[20]Effects of low temperature and low irradiance on the physiological characteristics and related gene expression of different pepper species. Wei, G.,Zhang, Z. Q.,Dai, X. Z.,Zou, X. X..

作者其他论文 更多>>

微信小程序