Rapid determination of amino acids in feedstuffs by high performance liquid chromatography using internal standard method with online pre-column derivatization

文献类型： 外文期刊

第一作者： Song Zhi-Feng

作者： Song Zhi-Feng;Li, Wang;Feng, Ji;Huang, Huang;Yu Zhi-Jing

作者机构：

关键词： high performance liquid chromatography;online pre-column derivatization;internal standard method;feedstuff;amino acid

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2007 年 35 卷 1 期

页码：

收录情况： SCI

摘要： A high performance liquid chromatographic method for rapid determination of amino acids in feedstuffs, was performed automatically by the autosampler, based on the online derivatization with o-phthaldialdehyde and 9-fluorenylmethyl chloroformate and the detection was carried out with diode array detector(for cystine and lysine) and fluorescence detector (for other amino acids except cystine lysine) combined a C-18 short column using gradient elution with norvaline as internal standard, adding to the samples before hydrolysis. The steps of sample preparation were simplified. The accuracy of determination of amino acids was improved without separating the interferents before analysis. The recoveries of standard spiking of 18 amino acids were in the range of 94. 1% - 102. 3%, except cystine, tryptophan (destroyed completely) and methionine (87. 3%). The relative standard deviations (RSD, n = 5) were in the range of 2. 1% - 4. 5%. The ratio of peak area of amino acids versus their concentration had a good linearity (correlation coefficients were 0. 9900 - 0. 9995) in the range of 4. 5 - 1000 mu mol/L. The limits of detection of the most amino acids were low, and sensitivities of detection were high. This method was suitable for rapid determination of amino acids in feedstuffs.

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