A new construct specific real-time PCR method for screening GMO ingredients with gat-tpinIl cassette in foods, feeds and seeds

文献类型： 外文期刊

第一作者： Chang, Li-Juan

作者： Chang, Li-Juan;Yin, Quan;Luo, Ping;Wang, Dong;Lei, Shao-Rong;Guo, Ling-An;Song, Jun;Liu, Wen-Juan;Deng, Qiang;Zhang, Fu-Li;Chang, Li-Juan;Yin, Quan;Luo, Ping;Wang, Dong;Lei, Shao-Rong;Guo, Ling-An;Song, Jun;Liu, Wen-Juan;Deng, Qiang;Niu, Bei

作者机构：

关键词： Construct-specific detection;Real-time PCR assay;GMO;Herbicide-resistance;Gat-tpinll cassette;Food products

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2018 年 86 卷

页码：

收录情况： SCI

摘要： With the number of genetically modified (GM) events authorized or pending for authorization yearly growing steadily, screening approaches need to be updated to enable full coverage and better discrimination of all these events. A construct-specific quantitative polymerase chain reaction (qPCR) assay for screening genetically modified organisms (GMO) with gat-tpinll cassette was developed in response to that need. The specificity of the built method was evaluated by testing commercial GM events and the sensitivity and repeatability were also assessed and validated. The limit of detection (LOD) could be as low as 5 copies, and the limit of quantification (LOQ) was 40 transgenic haploid genome copies. Three certified reference materials (CRMs) at known concentrations were analyzed as unknown samples to verify the developed real-time PCR system. And no substantial bias was shown with high accuracy. Thirty-five food products containing soybean, maize or canola were collected from the markets as practical samples and further validated the screening applicability of the built method. The results suggested that the method could be reliably used for identification of GM events with gat-tpinli construct in plant-derived samples. (C) 2017 Elsevier Ltd. All rights reserved.

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