Deep sequencing reveals high bacterial diversity and phylogenetic novelty in pit mud from Luzhou Laojiao cellars for Chinese strong-flavor Baijiu

文献类型： 外文期刊

第一作者： Tang, Yu-Ming

作者： Tang, Yu-Ming;Guo, Xiao-Jiao;Tian, Xin-Hui;Liu, Ying;Yao, Wan-Chun;Ren, Dao-Qun;Liu, Mao-Ke;Zhao, Ke;Zhang, Xiao-Ping;Deng, Bo

作者机构：

关键词： Baijiu;Luzhou Laojiao;16S rRNA gene;Phylogenetic novelty;Culture gap

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：6.475； 五年影响因子：6.508 ）

ISSN： 0963-9969

年卷期： 2017 年 102 卷

页码：

收录情况： SCI

摘要： The pit mud (PM) in fermentation cellar is a complex ecosystem that hosts diverse microbial communities that contribute to the production of Chinese strong-flavor Baijiu (CSFB). However, the microbial ecology of PM, particularly the extent of their phylogenetic novelty remains poorly understood. Here we conducted filmnina MiSeq sequencing to explore the diversity and novelty patterns of PM bacterial communities from Luzhou Laojiao cellars in use for 40 and 400 years. High diversity indices were found in the PM with 16 phyla and 105 genera. Interestingly, the compositions of dominant genera of the PM were significantly different than that reported previously for PM sampled from other geographic sites, suggesting greater microbial diversity of PM. The dominant genus of Caproiciproducens, a caproic acid-producing bacterium, is the first reported for Chinese Baijiu production. Our results demonstrate that the PM hosts a large number of novel taxa, with 26% of the total OTUs (operational taxonomic units) distant to cultured counterparts. The class Clostridia within Firmicutes presented the highest proportion of novel OTUs. Most novel OTUs were initially isolated from diverse environments, the most abundant of which came from Chinese Baijiu brewing ecosystems, highlighting the huge culturing gap within the PM, but at the same time suggesting the importance of these OTUs in CSFB production. The data presented in this study significantly increases the number of bacteria known to be associated with CSFB production and should help guide the future exploration of microbial resources for biotechnological applications.

分类号：

  • 相关文献

[1]A novel zoonotic Anaplasma species is prevalent in small ruminants: potential public health implications. Yang, Jifei,Liu, Zhijie,Niu, Qingli,Liu, Junlong,Han, Rong,Guan, Guiquan,Hassan, Muhammad Adeel,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong. 2017

[2]First molecular survey and identification of Anaplasma spp. in white yaks (Bos grunniens) in China. Yang, Jifei,Liu, Zhijie,Niu, Qingli,Liu, Junlong,Guan, Guiquan,Xie, Jingying,Luo, Jianxun,Yin, Hong,Wang, Shuqing,Wang, Shufang,Yin, Hong. 2016

[3]Streptomyces tritici sp nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.). Shi, Linlin,Li, Wenchao,Wang, Jiabin,Wang, Han,Tian, Yuanyuan,Xiang, Wensheng,Wang, Xiangjing,Xiang, Wensheng. 2018

[4]Paenibacillus thermophilus sp nov., a novel bacterium isolated from a sediment of hot spring in Fujian province, China. Zhou, Yu,Gao, Sheng,Yang, Ling-Ling,He, Jie,Zhang, Yun-Jiao,Tang, Shu-Kun,Li, Wen-Jun,Gao, Sheng,Yang, Ling-Ling,He, Jie,Zhang, Yun-Jiao,Tang, Shu-Kun,Li, Wen-Jun,Wei, Da-Qiao,Huang, Xing,Li, Wen-Jun. 2012

[5]Molecular survey and characterization of a novel Anaplasma species closely related to Anaplasma capra in ticks, northwestern China. Yang, Jifei,Liu, Zhijie,Niu, Qingli,Liu, Junlong,Han, Rong,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong,Shi, Yaoxu. 2016

[6]Illumina-based analysis of bacterial diversity related to halophytes Salicornia europaea and Sueada aralocaspica. Sh, Ying-wu,Lou, Kai,Li, Chun,Wang, Lei,Zhao, Zhen-yong,Zhao, Shuai,Tian, Chang-yan. 2015

[7]Anaplasma phagocytophilum in sheep and goats in central and southeastern China. Yang, Jifei,Liu, Zhijie,Niu, Qingli,Liu, Junlong,Han, Rong,Guan, Guiquan,Li, Youquan,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong. 2016

[8]Diversity of prokaryotes associated with soils around coal-fire gas vents in MaNasi county of Xinjiang, China. Lou, Kai,Zhang, Tao,Xu, Jianhua,Zeng, Jun. 2013

[9]Phylogenetic analysis of four Vibrio strains of pathogenic bacteria based on hemolysin genes and 16S rRNA genes. Wang, Xiuli,Yuan, Ye,Wang, Hongyue,Chang, Yaqing,Qiu, Xuemei,Zhou, Qunlan,Zhu, Jian,Cui, Yudong. 2009

[10]Phylogenetic analysis of epibacterial communities on the surfaces of four red macroalgae. Wu Hongqing,Liu Min,Zhang Wuchang,Xiao Tian,Wu Hongqing,Wu Hongqing,Liu Min. 2014

[11]Actinomadura jiaoheensis sp nov and Actinomadura sporangiiformans sp nov., two novel actinomycetes isolated from muddy soil and emended description of the genus Actinomadura. Zhao, Junwei,Guo, Lifeng,Sun, Pengyu,Han, Chuanyu,Bai, Lu,Liu, Chongxi,Li, Yunxi,Xiang, Wensheng,Wang, Xiangjing,Xiang, Wensheng.

[12]Streptomyces formicae sp nov., a novel actinomycete isolated from the head of Camponotus japonicus Mayr. Liu, Chongxi,Guo, Lifeng,Piao, Chenyu,Li, Zhilei,Li, Jiansong,Jia, Feiyu,Wang, Xiangjing,Xiang, Wensheng,Xiang, Wensheng.

[13]Micromonospora violae sp nov., isolated from a root of Viola philippica Car. Liu, Hui,Zhang, Xinhui,Wang, Shurui,Liu, Chongxi,Wang, Xiangjing,Xiang, Wensheng,Yu, Chao,Xiang, Wensheng.

[14]Kribbella qitaiheensis sp nov., a novel actinomycete isolated from soil. Zhao, Junwei,Liu, Chongxi,Han, Chuanyu,Bai, Lu,Sun, Pengyu,Li, Jiansong,Wang, Xiangjing,Xiang, Wensheng,Xiang, Wensheng.

[15]Lentibacillus amyloliquefaciens sp nov., a halophilic bacterium isolated from saline sediment sample. Wang, Jing-Li,Wang, Yan-Wei,Guo, Xiang,He, Ming-Xiong,Ma, Ke-Dong,Wang, Hui-Min,Zhou, Shan,Chen, Xiao-Rong,Kong, De-Long,Ruan, Zhi-Yong.

[16]Avoidance and Potential Remedy Solutions of Chimeras in Reconstructing the Phylogeny of Aphids Using the 16S rRNA Gene of Buchnera: A Case in Lachninae (Hemiptera). Chen, Rui,Wang, Zhe,Chen, Jing,Qiao, Ge-Xia,Chen, Rui,Wang, Zhe. 2015

[17]Catellatospora vulcania sp nov and Catellatospora paridis sp nov., two novel actinobacteria isolated from volcanic sediment and the rhizosphere of Paris polyphylla. Jia, Feiyu,Guo, Siyu,Shen, Yue,Gao, Meiyue,Liu, Chongxi,Zhou, Shuyu,Li, Jiansong,Guan, Xuejiao,Wang, Xiangjing,Xiang, Wensheng,Xiang, Wensheng.

[18]Paenibacillus vini sp nov., isolated from alcohol fermentation pit mud in Sichuan Province, China. Chen, Xiao-Rong,Wang, Yan-Wei,Wang, Hui-Min,Kong, De-Long,Guo, Xiang,Zhou, Yi-Qing,Ruan, Zhi-Yong,Chen, Xiao-Rong,Shao, Cheng-Bin,He, Ming-Xiong,Ma, Ke-Dong.

[19]Streptomyces castaneus sp nov., a novel actinomycete isolated from the rhizosphere of Peucedanum praeruptorum Dunn. Li, Zhilei,Bai, Lu,Yan, Kai,Zhao, Junwei,Lu, Chang,Liu, Chongxi,Wang, Xiangjing,Xiang, Wensheng,Xiang, Wensheng.

[20]Nocardia lasii sp nov., a novel actinomycete isolated from the cuticle of an ant (Lasius fuliginosus L). Bai, Lu,Ye, Lan,Zhao, Junwei,Yan, Kai,Xiang, Wensheng,Wang, Xiangjing,Bai, Lu,Xiang, Wensheng.

作者其他论文 更多>>

微信小程序