CONSTRUCTION OF EUKARYOTIC PLANT EXPRESSION VECTOR WITH THE SAG12 PROMOTER AND THE LEAF SENESCENCE-RELATED IPT GENE AND ITS GENETIC TRANSFORMATION IN RICE

文献类型： 外文期刊

第一作者： Qian, Qiufang

作者： Qian, Qiufang;Kang, Zhenhui;Wang, Guixue;Cai, Pingzhong;Zhang, Zhiyong

作者机构：

期刊名称：PAKISTAN JOURNAL OF BOTANY （ 影响因子：0.972； 五年影响因子：0.988 ）

ISSN： 0556-3321

年卷期： 2012 年 44 卷 6 期

页码：

收录情况： SCI

摘要： A pair of primers was designed according to the sequences published by GenBank to amplify the isopentenyl transferase gene (ipt gene) of the plasmid in Agrobacterium tumefaciens (strain C58). The 5'-untranslated sequence of the senescence-associated SAG12 gene of Arabidopsis was isolated by the polymerase chain reaction (PCR). In addition, we constructed the plant-expression vectors (pBI121-ipt and pCAMBIA1301-SAG12-ipt), which were regulated by the SAG12 promoter and the ipt gene. The plant-expression vector was used to transform the embryos of rice cultivar Zhonghua 16 with the help of the A. tumefaciens system. Plantlets were regenerated in vitro by selection on medium containing hygromycin. PCR amplification showed that the target gene was successfully integrated into the transformed plants.

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