Isolation of a new repetitive DNA sequence from Secale africanum enables targeting of Secale chromatin in wheat background

文献类型： 外文期刊

第一作者： Yang, Zu-Jun

作者： Yang, Zu-Jun;Li, Guang-Rong;Zeng, Zi-Xian;Zhang, Yong;Zhou, Jian-Ping;Liu, Zhao-Hui;Ren, Zheng-Long

作者机构：

关键词： Secale africanum;rye;molecular marker;FISH;chromatin detection

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2008 年 159 卷 1-2 期

页码：

收录情况： SCI

摘要： A genome specific DNA sequence that detects Secale africanum chromatin incorporated into wheat was developed in this study. Random amplified polymorphic DNA (RAPD) analysis was used to search for genome specific DNA sequences of S. africanum in lines, R111, "mianyang11" (MY11) and wheat-rye 1RS/1BL translocations R25 and R57. A high copy rye-specific DNA segment pSaD15(940) of the S. africanum genome was obtained. The sequence of pSaD15 did not show any significant homology to other reported sequences in databases and it is therefore a new repetitive sequence of Secale. PCR primers were designed for pSaD15(940), which amplify a clear 887 bp fragment in S. africanum but not in any wheat. The primers also amplified an 887 bp fragment in other accessions of rye, Chinese Spring-Imperial rye chromosome additions and a diverse range of material carrying different rye chromosomes or chromosomal segments. In situ hybridization showed that probe pSaD15(940) was specifically hybridized throughout all rye chromosomes arms except for the terminal regions. The advantage of the rye-specific probe developed herein compared to those of previous reports is that it has been shown to be widely applicable to other Secale species. The probe will be useful as a molecular marker for the introgression of S. africanum and other rye chromosome segments into the wheat genome.

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