Pseudomonas Cyclic Lipopeptide Medpeptin: Biosynthesis and Modulation of Plant Immunity

文献类型： 外文期刊

第一作者： Gu, Yi-Lin

作者： Gu, Yi-Lin;Li, Jun-Zhou;Cong, Shen;Wang, Jing;Ma, Yi-Nan;Wei, Hai -Lei;Li, Yan

作者机构： Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, State Key Lab Efficient Utilizat Arid & Semiarid A, Minist Agr & Rural Affairs,Key Lab Microbial Resou, Beijing 100081, Peoples R China;Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

关键词： Pseudomonas; Cyclic lipopeptide; Cell death; Leucine-rich repeat extension (LRX); Medpeptin; Receptor -like kinase (RLK)

期刊名称：ENGINEERING （ 2022影响因子：12.8； 五年影响因子：13.6 ）

ISSN： 2095-8099

年卷期： 2023 年 28 卷

页码：

收录情况： SCI

摘要： The multifunctional secondary metabolites known as cyclic lipopeptides (CLPs), which are produced by a large variety of bacteria, have become a key category of plant immunity elicitors. Pseudomonas-CLPs (PsCLPs) are extremely diverse in structure and biological activity. However, an understanding of CLP-plant structure-function interactions currently remains elusive. Here, we identify medpeptin, a novel CLP from Pseudomonas mediterranea that consists of 22 amino acids. Medpeptin is synthesized by a non-ribosomal peptide synthase (NRPS) gene cluster and regulated by a quorum-sensing system. Further research indicates that medpeptin does not exhibit antimicrobial activity; instead, it induces plant cell death immunity and confers resistance to bacterial infection. Comparative transcriptome analysis and virus-induced gene silencing (VIGS) reveal a set of immune signaling candidates involved in medpeptin perception. Silencing of a cell-wall leucine-rich repeat extensin protein (NbLRX3) or a receptor-like protein kinase (NbRLK25)-but not BAK1 or SGT1-compromises medpeptin-triggered cell death and resistance to pathogen infection in Nicotiana benthamiana. Our findings point to a noncanonical mechanism of CLP sensing and suggest perspectives for the development of plant disease resistance. (c) 2023 THE AUTHORS. Published by Elsevier LTD on behalf of Chinese Academy of Engineering and Higher Education Press Limited Company. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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