Functional defect at the rice choline monooxygenase locus from an unusual post-transcriptional processing is associated with the sequence elements of short-direct repeats

文献类型: 外文期刊

第一作者: Niu, Xiangli

作者: Niu, Xiangli;Wang, Yuguo;Zheng, Wenjing;Chang, Lijuan;Wang, Qilin;Wei, Xin;Yu, Guirong;Lu, Bao-Rong;Liu, Yongsheng

作者机构:

关键词: alternative splicing;choline monooxygenase;glycine betaine;Oryza sativa (rice);short-direct repeats

期刊名称:NEW PHYTOLOGIST ( 影响因子:10.151; 五年影响因子:10.475 )

ISSN: 0028-646X

年卷期: 2007 年 175 卷 3 期

页码:

收录情况: SCI

摘要: Glycine betaine (GB), a quaternary ammonium solute, plays a crucial role in developing osmotic tolerance. Rice contains a choline monooxygenase (CMO) and two betaine aldehyde dehydrogenase homologues that are required for GB synthesis, but usually no GB is accumulated in rice (Oryza sativa). To elucidate the molecular processes that underlie the GB deficiency in rice, an experiment involving rice and spinach (Spinacia oleracea) was conducted to analyze the products transcribed from CMO genes. Reverse transcription- polymerase chain reaction (RT-PCR) was used to obtain CMO transcripts and a sequencing approach was employed to analyze the structural composition of various CMO transcripts. The results showed that most rice CMO transcripts were processed incorrectly, retaining introns or deleted of coding sequences; the unusual deletion events occurred at sequence elements of the short-direct repeats. In conclusion, the production of incorrect CMO transcripts results in a deficiency of the full-length CMO protein and probably reduces GB accumulation considerably in rice plants. Sequence comparison results also implied that the unusual deletion-site selection might be mediated by the short-direct repeats in response to stress conditions.

分类号:

  • 相关文献

[1]An unusual posttranscriptional processing in two Betaine aldehyde dehydrogenase loci of cereal crops directed by short, direct repeats in response to stress conditions. Zheng, Wenjing,Lu, Bao-Rong,Ren, Guangjun,Huang, Weizao,Wang, Songhu,Liu, Junli,Tang, Zizhi,Luo, Di,Wang, Yuguo,Liu, Yongsheng.

[2]Auxin response factor (OsARF12), a novel regulator for phosphate homeostasis in rice (Oryza sativa). Wang, SuiKang,Zhang, SaiNa,Sun, ChenDong,Xu, YanXia,Chen, Yue,Yu, ChenLiang,Jiang, De-An,Qi, YanHua,Qian, Qian.

[3]Jasmonic acid-mediated defense suppresses brassinosteroid-mediated susceptibility to Rice black streaked dwarf virus infection in rice. He, Yuqing,He, Yuqing,Zhang, Hehong,Sun, Zongtao,Li, Junmin,Hong, Gaojie,Yan, Fei,Chen, Jianping,Zhang, Hehong,Zhu, Qisong,Zhou, Xuebiao,MacFarlane, Stuart.

[4]Involvement of abscisic acid and ethylene in the responses of rice grains to water stress during filling. Yang, JC,Zhang, JH,Ye, YX,Wang, ZQ,Zhu, QS,Liu, LJ. 2004

[5]Chemically enhanced lipid production from microalgae under low sub-optimal temperature. Wang, Yuancong,He, Bing,Chen, Yi-Feng,Sun, Zhilan. 2016

[6]Amelioration of postharvest chilling injury in sweet pepper by glycine betaine. Gao, Lipu.

[7]Effect of exogenous glycine betaine on qualities of button mushrooms (Agaricus bisporus) during postharvest storage. Wang, Zhaogai,Chen, Lijuan,Yang, Hui,Wang, Anjian.

[8]Differential responses of lipid peroxidation and antioxidants in Alternanthera philoxeroides and Oryza sativa subjected to drought stress. Gao, Jianming,Xiao, Qiang,Ding, Liping,Chen, Mingjie,Yin, Liang,Li, Jinzhi,Zhou, Shiyi,He, Guangyuan,Gao, Jianming.

[9]SpBADH of the halophyte Sesuvium portulacastrum strongly confers drought tolerance through ROS scavenging in transgenic Arabidopsis. Yang, Chenglong,Zhou, Yang,Fan, Jie,Shen, Longbin,Yao, Yuan,Li, Ruimei,Fu, Shaoping,Duan, Ruijun,Guo, Jianchun,Yang, Chenglong,Zhou, Yang,Fan, Jie,Shen, Longbin,Yao, Yuan,Li, Ruimei,Fu, Shaoping,Duan, Ruijun,Guo, Jianchun,Yang, Chenglong,Fu, Yuhua,Zhou, Yang,Fan, Jie,Hu, Xinwen.

[10]Mechanisms of glycine betaine enhancing oxidative stress tolerance and biocontrol efficacy of Pichia caribbica against blue mold on apples. Zhang, Xiaoyun,Yang, Qiya,Zhao, Lina,Apaliya, Maurice Tibiru,Zhang, Hongyin,Zhang, Guochao,Chen, Keping,Li, Pengxia,Gu, Xiangyu.

[11]Water stress induces in pear leaves the rise of betaine level that is associated with drought tolerance in pear. Gao, XP,Yan, JY,Liu, EK,Shen, YY,Lu, YF,Zhang, DP.

[12]A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression. Li, H.,Jiang, Q.,Ju, Z. H.,Huang, J. M.,Wang, X. G.,Yang, C. H.,Sun, Y.,Zhong, J. F.,Wang, C. F.,Li, H.,Yang, G. W.. 2016

[13]A SNP in intron 8 of CD46 causes a novel transcript associated with mastitis in Holsteins. Wang, Xiuge,Zhong, Jifeng,Gao, Yundong,Ju, Zhihua,Huang, Jinming,Huang, Jinming. 2014

[14]Increased expression of a novel splice variant of the complement component 4 (C4A) gene in mastitis-infected dairy cattle. Yang, Y.,Huang, J. M.,Ju, Z. H.,Li, Q. L.,Zhou, L.,Li, R. L.,Li, J. B.,Zhong, J. F.,Wang, C. F.,Yang, Y.,Shi, F. X.,Yang, Y.. 2012

[15]Two splice variants of the bovine lactoferrin gene identified in Staphylococcus aureus isolated from mastitis in dairy cattle. Huang, J. M.,Wang, Z. Y.,Ju, Z. H.,Wang, C. F.,Li, Q. L.,Sun, T.,Hou, Q. L.,Hang, S. Q.,Hou, M. H.,Zhong, J. F.. 2011

[16]Novel splice variants of the bovine PCK1 gene. Zhang, Z. B.,Zhao, Z. S.,Huang, J. M.,Zhang, Z. B.,Zhang, W.,Li, R. L.,Li, J. B.,Zhong, J. F.. 2013

[17]Alternative splicing and mRNA expression analysis of bovine SLAMF7 gene in healthy and mastitis mammary tissues. Ju, Zhihua,Wang, Changfa,Li, Qiuling,Hou, Minghai,Gao, Shuai,Hou, Qinlei,Li, Jianbin,Huang, Jinming,Zhong, Jifeng.

[18]The comparison of alternative splicing among the multiple tissues in cucumber. Sun, Ying,Song, Hongtao,Lin, Kui,Pang, Erli,Sun, Ying,Song, Hongtao,Lin, Kui,Pang, Erli,Hou, Han,Zhang, Zhonghua,Hou, Han,Hu, Jinglu. 2018

[19]Cloning and Characterization of New Splice Variants of Insulin-Like Growth Factor-1 Gene in Songliao Black Pig. Liu, Chang,Zhaoa, Zhihui,Bai, Wenlin,Sun, Boxing,Zhang, Yonghong,Yang, Runjun,Liu, Chang,Bai, Wenlin. 2012

[20]Apple NPR1 homologs and their alternative splicing forms may contribute to SA and disease responses. Zhang, Junke,Wei, Qinping,Zhang, Junke,Wei, Qinping,Jiao, Peng,Zhang, Chong,Tong, Xiaolei,Xu, Lingfei. 2016

作者其他论文 更多>>