Extracellular vesicles from Fasciola gigantica induce cellular response to stress of host cells

文献类型： 外文期刊

第一作者： Guo, Aijiang

作者： Guo, Aijiang;Wang, Li;Meng, Xuelian;Zhang, Shaohua;Luo, Xuenong;Wang, Shuai;Cai, Xuepeng;Guo, Aijiang;Luo, Xuenong;Wang, Li;Sheng, Zhaoan;Huang, Weiyi

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关键词： Fasciola gigantica; Extracellular vesicles; Human intrahepatic biliary epithelial cells; Reactive oxygen species; Stress

期刊名称：EXPERIMENTAL PARASITOLOGY （ 影响因子：2.132； 五年影响因子：2.198 ）

ISSN： 0014-4894

年卷期： 2021 年 231 卷

页码：

收录情况： SCI

摘要： Extracellular vesicles (EVs) from parasitic helminths play an important role in immunomodulation. However, EVs are little studied in the important parasite Fasciola gigantica. Here the ability of EVs from F. gigantica to induce cellular response to stress (reactive oxygen species generation, autophage and DNA damage response) in human intrahepatic biliary epithelial cells (HIBEC) was investigated. F. gigantica-derived EVs were isolated by ultracentrifugation, and identified with transmission electron microscopy, nanoparticle size analysis and parasite-derived EV markers. Internalization of EVs by HIBEC was determined by confocal immunofluorescence microscopy and flow cytometry. ROS levels in HIBEC were detected by molecular probing. EVs-induced autophagy and DNA-damaging effects were determined by evaluating expression levels of light chain 3B protein (LC3B), phosphor-H2A.X and phosphor-Chk1, respectively. Results revealed that EVs with sizes predominately ranging from 39 to 110 nm in diameter were abundant in adult F. gigantica and contained the parasite-derived marker proteins enolase and 14-3-3, and EVs were internalized by HIBEC. Further, uptake of EVs into HIBEC was associated with increased levels of reactive oxygen species, LC3II, phosphor-H2A.X and phosphor-Chk1, suggesting EVs are likely to induce autophagy and DNA damage & repair processes. These results indicate F. gigantica EVs are associated with modulations of host cell responses and have a potential important role in the host-parasite interactions.

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