Purification, characterization and immunomodulatory activity of water extractable polysaccharides from the swollen culms of Zizania latifolia
文献类型: 外文期刊
第一作者: Wang, Mingchun
作者: Wang, Mingchun;Zhao, Shiwei;Zhu, Peilei;Nie, Chenzhipeng;Ma, Shuping;Wang, Naifu;Du, Xianfeng;Zhou, Yibin;Wang, Mingchun;Zhao, Shiwei;Zhu, Peilei;Nie, Chenzhipeng;Ma, Shuping;Wang, Naifu;Du, Xianfeng;Zhou, Yibin;Zhu, Peilei
作者机构:
关键词: Polysaccharide;Zizania latifolia;Purification;Characterization;Immunomodulatory activity
期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:6.953; 五年影响因子:6.737 )
ISSN: 0141-8130
年卷期: 2018 年 107 卷
页码:
收录情况: SCI
摘要: The swollen culms of Zizania latifolia is a popular vegetable and traditional herbal medicine mainly consumed in some Asian countries. In our previous study, water extractable polysaccharide (ZLPs-W) and alkali extractable polysaccharide (ZLPs-A) was sequentially prepared from the swollen culms of Zizania latifolia. In vitro cell assay revealed that ZLPs-W without cytotoxicity had higher immunomodulatory activity than ZLPs-A. Therefore, in this study, ZLPs-W was further separated to three purified polysaccharides of ZLPs-W1, ZLPs-W2, and ZLPs-W3 by anion exchange chromatography and size exclusive chromatography in sequence. They were found to be homogeneous polysaccharides in beta-type glycosidic linkage. ZLPs-W1 and ZLPs-W2 without triple helix conformation mainly contained Glc in molar percentage of 96.47% and 85.1%, respectively. ZLPs-W3 with triple helix conformation mainly consisted of Man, Rha, GalA, Glc, Gal and Ara in the molar ratio of 10.23:14.14:37.44:17.14:16.54:4.51. In addition, all the three purified polysaccharides could effectively enhanced the proliferation, phagocytosis, and nitric oxide production of murine macrophage RAW 264.7, suggesting that they possessed a potent immunostimulatory activity and could be developed as immunomodulator in medicine or functional food areas. (C) 2017 Elsevier B.V. All rights reserved.
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