Characterization and analysis of CCR and CAD gene families at the whole-genome level for lignin synthesis of stone cells in pear (Pyrus bretschneideri) fruit

文献类型： 外文期刊

第一作者： Cheng, Xi

作者： Cheng, Xi;Li, Manli;Li, Dahui;Zhang, Jinyun;Jin, Qing;Sheng, Lingling;Cai, Yongping;Lin, Yi;Zhang, Jinyun

作者机构：

关键词： Pyrus bretschneideri;Stone cells;Lignin synthesis;CCR and CAD gene families;Expression analysis;Subcellular localization

期刊名称：BIOLOGY OPEN （ 影响因子：2.422； 五年影响因子：2.647 ）

ISSN： 2046-6390

年卷期： 2017 年 6 卷 11 期

页码：

收录情况： SCI

摘要： The content of stone cells has significant effects on the flavour and quality of pear fruit. Previous research suggested that lignin deposition is closely related to stone cell formation. In the lignin biosynthetic pathway, cinnamoyl-CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD), dehydrogenase/reductase family members, catalyse the last two steps in monolignol synthesis. However, there is little knowledge of the characteristics of the CCR and CAD families in pear and their involvement in lignin synthesis of stone cells. In this study, 31 CCRs and 26 CADs were identified in the pear genome. Phylogenetic trees for CCRs and CADs were constructed; key amino acid residues were analysed, and three-dimensional structures were predicted. Using quantitative real-time polymerase chain reaction (qRT-PCR), PbCAD2, PbCCR1, -2 and-3 were identified as participating in lignin synthesis of stone cells in pear fruit. Subcellular localization analysis showed that the expressed proteins (PbCAD2, PbCCR1, -2 and-3) are found in the cytoplasm or at the cell membrane. These results reveal the evolutionary features of the CCR and CAD families in pear as well as the genes responsible for regulation of lignin synthesis and stone cell development in pear fruit.

分类号：

  • 相关文献

[1]Expression of an alfalfa (Medicago sativa L.) ethylene response factor gene MsERF8 in tobacco plants enhances resistance to salinity. Chen, Tingting,Sun, Yan,Chen, Tingting,Yang, Qingchuan,Kang, Junmei,Ding, Wang,Zhang, Tiejun,Chen, Tingting,Zhang, Xinquan,Gruber, Margaret.

[2]An alfalfa (Medicago sativa L.) ethylene response factor gene, MsERF11, enhances salt tolerance in transgenic Arabidopsis. Chen, Tingting,Yang, Qingchuan,Ding, Wang,Chen, Tingting,Zhang, Xinquan,Gruber, Margaret. 2012

[3]Jasmonic acid is involved in the water-stress-induced betaine accumulation in pear leaves. Gao, XP,Wang, XF,Lu, YF,Zhang, LY,Shen, YY,Liang, Z,Zhang, DP. 2004

[4]Study of the structure and biosynthetic pathway of lignin in stone cells of pear. Cai, Yongping,Li, Guoqing,Nie, Jingquan,Lin, Yi,Nie, Fan,Zhang, Jinyun,Xu, Yiliu. 2010

[5]Evidence supporting the involvement of MHO in the formation of superficial scald in Dangshansuli' pears. Hui, Wei,Niu, Junpeng,Xu, Xiaoyan,Guan, Junfeng.

[6]Molecular cloning and expression analysis of FvMYB1 from Fraxinus velutina Torr.. Li, Tian,Bi, Yu-Ping,Li, Tian,Peng, Zhen-Ying,Bi, Yu-Ping,Fan, Zhong-Xue,Li, Tian,Peng, Zhen-Ying,Bi, Yu-Ping,Fan, Zhong-Xue,Li, Tian. 2013

[7]Genome-wide identification of Thellungiella salsuginea microRNAs with putative roles in the salt stress response. Zhang, Quan,Sun, Wei,Liu, Yan,Wang, Xingjun,Zhao, Yanxiu,Zhao, Chuanzhi,Li, Ming,Xia, Han,Sun, Mingnan,Li, Aiqin,Li, Changsheng,Zhao, Shuzhen,Hou, Lei,Picimbon, Jean-Francois,Wang, Xingjun. 2013

[8]Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene. Zhang, Yue,Wang, Xingjun,Zhang, Yue,Xia, Han,Zhao, Chuanzhi,Li, Aiqin,Wang, Xingjun,Yuan, Mei. 2012

[9]Genomic organization, differential expression, and functional analysis of the SPL gene family in Gossypium hirsutum. Zhang, Xiaohong,Dou, Lingling,Pang, Chaoyou,Song, Meizhen,Wei, Hengling,Fan, Shuli,Wang, Chengshe,Yu, Shuxun. 2015

[10]Genome-wide identification and functional analysis of the TIFY gene family in response to drought in cotton. Ge Zhao,Yun Song,Caixiang Wang,Hamama Islam Butt,Qianhua Wang,Chaojun Zhang,Zuoren Yang,Zhao Liu,Eryong Chen,Xueyan Zhang,Fuguang Li.

[11]Genome-wide identification and characterization of SnRK2 gene family in cotton (Gossypium hirsutum L.). Zhao Liu,Xiaoyang Ge,Zuoren Yang,Li, Fuguang,Zhang, Xueyan,Chaojun Zhang,Ge Zhao,Eryong Chen,Ji Liu,Xueyan Zhang,Fuguang Li. 2017

[12]Comparative Transcriptomic Analysis Reveals a Series of Single Nucleotide Polymorphism between Redand White-fleshed Loquats (Eriobotrya japonica). Li, Jing,Chen, Dong,Xie, Hongjiang,Tu, Meiyan,Jia, Liu,Jiang, Guoliang,Sun, Shuxia,Li, Jing,Chen, Dong,Xie, Hongjiang,Tu, Meiyan,Jia, Liu,Jiang, Guoliang. 2017

[13]Genome-Wide Identification and Expression Profile of Dof Transcription Factor Gene Family in Pepper (Capsicum annuum L.). Wu, Zhiming,Bang, Guansheng,Cheng, Jiaowen,Cui, Junjie,Hu, Kailin,Xu, Xiaowan,Luo, Xirong,Chen, Xiaocui,Tang, Xiangqun,Qin, Cheng,Qin, Cheng. 2016

[14]Genome-wide analysis of the maize (Zea may L.) CPP-like gene family and expression profiling under abiotic stress. Song, X. Y.,Zhang, Y. Y.,Wu, F. C.,Zhang, L.. 2016

[15]Molecular Characterization, Gene Evolution, and Expression Analysis of the Fructose-1, 6-bisphosphate Aldolase (FBA) Gene Family in Wheat (Triticum aestivum L.). Lv, Geng-Yin,Guo, Xiao-Guang,Xie, Li-Ping,Xie, Chang-Gen,Zhang, Xiao-Hong,Yang, Yuan,Xiao, Lei,Tang, Yu-Ying,Guo, Ai-Guang,Xu, Hong,Xie, Chang-Gen,Guo, Ai-Guang,Xu, Hong,Pan, Xing-Lai. 2017

[16]Genome-wide identification and expression analysis of the CPP-like gene family in soybean. Zhang, L.,Wang, Y. M.,Yuan, C. P.,Zhang, Y. Y.,Li, H. Y.,Dong, Y. S.,Zhao, H. K.,Yan, X. F.,Li, Q. Y.. 2015

[17]Molecular Cloning, Characterization and Expression Analysis of Duck Tyrosinase-Related Protein-1. Wang, Cui,Yu, Huawen,Peng, Xiuli,Feng, Yanping,Gong, Yanzhang,Li, Shijun,Liang, Zhenhua. 2010

[18]Genomic, evolutionary and expression profile analysis of Hsp70 superfamily in A and D genome of cotton (Gossypium spp.) under the challenge of Verticillium dahliae. Xiao, Songhua,Yu, Jingzhong,Yu, Deyue,Xiao, Songhua,Xu, Jianwen,Zhao, Jun,Liu, Jianguang,Wu, Qiaojuan. 2017

[19]Identification and expression pattern of a glutathione S-transferase in Echinochloa crus-galli. Li, G.,Wu, S. G.,Yu, R. X.,Cang, T.,Chen, L. P.,Zhao, X. P.,Cai, L. M.,Wu, C. X.. 2013

[20]Identifying suitable reference genes for gene expression analysis in developing skeletal muscle in pigs. Niu, Guanglin,Yang, Yalan,Zhang, YuanYuan,Hua, Chaoju,Wang, Zishuai,Tang, Zhonglin,Li, Kui,Niu, Guanglin,Yang, Yalan,Tang, Zhonglin,Li, Kui. 2016

作者其他论文 更多>>

微信小程序