Comparative transcriptomic and proteomic analyses of midrib and root of Wanjincheng orange (Citrus sinensis Osbeck) in response to the early invasion of Candidatus Liberibacter asiaticus

文献类型： 外文期刊

第一作者： Xie, Zhu

作者： Xie, Zhu;Wang, Shuai;Ma, Juanjuan;Dong, Liting;Qu, Rongrong;He, Yong-Rui;Chen, Shanchun;Zou, Xiuping;Xie, Zhu

作者机构： Southwest Univ, Chinese Acad Agr Sci, Citrus Res Inst, Chongqing 400716, Peoples R China;Nanjing Agr Univ, Coll Hort, Nanjing, Peoples R China

关键词： Citrus sinensis; Huanglongbing; Transcriptomic; Proteomic; Association analysis

期刊名称：SCIENTIA HORTICULTURAE （ 2022影响因子：4.3； 五年影响因子：4.5 ）

ISSN： 0304-4238

年卷期： 2023 年 318 卷

页码：

收录情况： SCI

摘要： Citrus Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (CLas), is the most destructive disease of citrus plant. HLB can harm almost all citrus varieties and no cure is currently available. Our previous study reported that Wanjincheng orange (Citrus sinensis Osbeck) leaf and root exhibit significant difference in response to CLas infection using leaf-disk grafting. In this study, we used a multiomic approach (proteomics and tran-scriptomics) to identify nodes that can help in explaining these differences and ultimately contribute to the understanding of the response of midrib and root in the early invasion of CLas. The results of comparative transcriptomic analysis displayed that 2,782 differentially expressed genes (DEGs) were upregulated and 2,807 were downregulated. Meanwhile, the results of comparative proteomic analysis displayed that 226 and 182 differentially expressed proteins (DEPs) were up-and downregulated by CLas, respectively. Transcriptomic and proteomic association analyses revealed 2 downregulated and 6 upregulated genes in midrib and root. The analysis of DEGs and DEPs revealed that secondary metabolites, and detoxification pathways played important roles in response to the early invasion of CLas. Moreover, nitrogen metabolism in midrib and root was influenced by CLas. This study revealed the transcriptomic and proteomic differences between midrib and root in Wan-jincheng orange during early invasion of CLas and provided some potential genes and proteins for breeding HLB-tolerant citrus in future.

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