Comparative analysis of different protocols for extraction of DNA from fish scales of Cyprinus carpio
文献类型: 外文期刊
第一作者: Li, Yanhe
作者: Li, Yanhe;Gul, Yasmeen;Cui, Lei;Cao, Xiaojuan;Wang, Weimin;Li, Yanhe;Gul, Yasmeen
作者机构:
关键词: Cyprinus carpio;DNA extraction;fish scales;PCR
期刊名称:INDIAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.414; 五年影响因子:0.542 )
ISSN: 0972-5849
年卷期: 2015 年 14 卷 3 期
页码:
收录情况: SCI
摘要: Four DNA extraction protocols from the fish scales of Cyprinus carpio were compared. The DNAs obtained from all the protocols were tested by PCR and the DNAs extracted using protocol 1 and 2 were also tested by restriction analysis. The results indicated that all four protocols extracted DNA suitable for PCR amplification. However, variations in the yield and purity of extracted DNA were observed between protocols. The DNA samples prepared by protocol 1 and 2 were amenable to restriction digestion by restriction enzyme HindIII and NotI. Different protocols have their own merits and shortcomings. Thus, the present study can be used as a reference for selecting suitable protocol according to the aim and need of the study undertaken.
分类号:
- 相关文献
作者其他论文 更多>>
-
Spatial heterogeneity determines the gastrointestinal microbiome signatures and ecological processes that govern bacterial community assembly in sheep
作者:Zhang, Yukun;Cheng, Jiangbo;Li, Fadi;Zhang, Deyin;Yang, Xiaobin;Xu, Dan;Zhao, Yuan;Zhao, Liming;Li, Xiaolong;Tian, Huibin;Weng, Xiuxiu;Wang, Weimin;Zhang, Yukun;Cheng, Jiangbo;Li, Fadi;Zhang, Deyin;Yang, Xiaobin;Xu, Dan;Zhao, Yuan;Zhao, Liming;Li, Xiaolong;Tian, Huibin;Weng, Xiuxiu;Wang, Weimin;Zhang, Yukun;Cheng, Jiangbo;Li, Fadi;Zhang, Deyin;Yang, Xiaobin;Xu, Dan;Zhao, Yuan;Zhao, Liming;Li, Xiaolong;Tian, Huibin;Weng, Xiuxiu;Wang, Weimin;Zhang, Yukun;Cheng, Jiangbo;Li, Fadi;Zhang, Deyin;Yang, Xiaobin;Xu, Dan;Zhao, Yuan;Zhao, Liming;Li, Xiaolong;Tian, Huibin;Weng, Xiuxiu;Wang, Weimin;Lin, Changchun;Zhang, Xiaoxue;Li, Chong
关键词:sheep; gastrointestinal tract; co-occurrence networks; enterotypes; bacterial community assembly
-
Ovine TUSC5 and IGFBP3 Gene Polymorphisms and their Association with Tail Fat Weight in Sheep
作者:Cao, Peiliang;He, Lijuan;Jia, Guoxing;Kong, Dewen;Li, Linting;Zhang, Jian;Li, Hongjian;Zhang, Xiaoxue;Zhang, Deyin;Xu, Dan;Ma, Zongwu;Pu, Mengru;Li, Fadi;Tian, Huibin;Wang, Weimin;Wu, Weiwei
关键词:IGFBP3; sheep; SNPs; TUSC5; tail fat weight
-
Cytogenetics and genomics analysis of cold-hardy perennial wheatgrass: insights into agronomic performance, chromosome composition, and gene expression
作者:Zhao, Xinyu;Ding, Jiarui;Wu, Chunfei;Song, Anning;Song, Weiwei;Liu, Meng;Yang, Donghe;Liu, Yizhuo;He, Miao;Xing, Qianyu;Zhang, Huixin;Li, Xinling;Zhang, Yanming;Zhang, Zhimeng;Xie, Jingzhong;He, Fei;Zhang, Zhimeng;He, Fei;Qu, Yunfeng;Li, Hongjie;Zhao, Haibin;Cui, Lei;He, Fei
关键词:
Thinopyrum intermedium ; perennial wheatgrass; sequential multicolor genomic hybridization; RNA-seq; single-nucleotide polymorphism; gene expression; distant hybrid breeding -
A modified method for the simultaneous determination of fluindapyr and its metabolites residues in vegetables and fruits by SPE and UHPLC-MS/ MS
作者:Han, Wei;Tang, Hongxia;Li, Yubo;Men, Dianying;Dong, Maofeng;Wang, Weimin;Zhao, Li;Han, Lijun
关键词:Fluindapyr; Metabolites; UHPLC-MS/MS; Method validation; Residues
-
Characterization of a Natural Accession of Elymus sibiricus with In Situ Hybridization and Agronomic Evaluation
作者:Liu, Yizhuo;Ding, Jiarui;Wu, Chunfei;Song, Weiwei;Zhao, Xinyu;Li, Mingyao;Yan, Xinyu;Zhu, Liangyu;Bao, Yaqi;Liu, Dianhao;Li, Xinling;Zhang, Yanming;Zhao, Haibin;Jin, Hui;Zhang, Rui;Qu, Yunfeng;Li, Hongjie;Cui, Lei
关键词:
Elymus ; smGISH; karyotype; chromosome; cold-hardiness -
Metabolic response of Sinosolenaia oleivora to heat and drought stress using a quasi-targeted metabolomics approach
作者:Ma, Xueyan;Jin, Wu;Wang, Liufu;Chen, Wanwen;Wang, Yufeng;Wen, Haibo;Ma, Xueyan;Jin, Wu;Wang, Liufu;Chen, Wanwen;Wang, Yufeng;Wen, Haibo;Ma, Xueyan;Cao, Xiaojuan
关键词:Metabolic response; Metabolome; Heat stress; Drought stress; Freshwater mussel
-
Application of multi-omics technology in pathogen identification and resistance gene screening of sheep pneumonia
作者:Huang, Kai;Li, Xiaolong;Xu, Dan;Tian, Huibin;Li, Fadi;Wang, Weimin;Huang, Kai;Li, Xiaolong;Xu, Dan;Tian, Huibin;Li, Fadi;Wang, Weimin;Huang, Kai;Li, Xiaolong;Xu, Dan;Tian, Huibin;Li, Fadi;Wang, Weimin;Yuan, Lvfeng;Liu, Jia;Zhang, Xiaoxue;Peng, Jie
关键词:Sheep; Multi-omics; Pathogen identification;
Mycoplasma ovipneumoniae ; Genome-wide association study; Disease-resistant breeding