Comparative analysis of different protocols for extraction of DNA from fish scales of Cyprinus carpio

文献类型: 外文期刊

第一作者: Li, Yanhe

作者: Li, Yanhe;Gul, Yasmeen;Cui, Lei;Cao, Xiaojuan;Wang, Weimin;Li, Yanhe;Gul, Yasmeen

作者机构:

关键词: Cyprinus carpio;DNA extraction;fish scales;PCR

期刊名称:INDIAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.414; 五年影响因子:0.542 )

ISSN: 0972-5849

年卷期: 2015 年 14 卷 3 期

页码:

收录情况: SCI

摘要: Four DNA extraction protocols from the fish scales of Cyprinus carpio were compared. The DNAs obtained from all the protocols were tested by PCR and the DNAs extracted using protocol 1 and 2 were also tested by restriction analysis. The results indicated that all four protocols extracted DNA suitable for PCR amplification. However, variations in the yield and purity of extracted DNA were observed between protocols. The DNA samples prepared by protocol 1 and 2 were amenable to restriction digestion by restriction enzyme HindIII and NotI. Different protocols have their own merits and shortcomings. Thus, the present study can be used as a reference for selecting suitable protocol according to the aim and need of the study undertaken.

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