Effects of exogenous nitric oxide on antioxidation and DNA methylation of Dendrobium huoshanense grown under drought stress

文献类型： 外文期刊

第一作者： Fan, Honghong

作者： Fan, Honghong;Li, Tingchun;Guan, Lei;Li, Zhengpeng;Guo, Ning;Cai, Yongping;Lin, Yi;Li, Tingchun

作者机构：

关键词： Dendrobium huoshanense;Exogenous nitric oxide;Methylation sensitive amplified polymorphism (MSAP);Drought stress;DNA methylation;Sodium nitroprusside (SNP)

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN： 0167-6857

年卷期： 2012 年 109 卷 2 期

页码：

收录情况： SCI

摘要： Nitric oxide (NO) is an important biological messenger in plants, which has been implicated in response to abiotic stress. To study the effects of exogenous NO on drought menace, the tube seedlings of Dendrobium huoshanense were selected and treated with 10% polyethylene glycol (PEG-6000) to simulate drought stress. After application of sodium nitroprusside (SNP), the relative water content (RWC) and antioxidant enzyme activities were determined. As a result, plant treated with 50 mu mol L-1 of SNP maintained high level of RWC and lower content of malondialdehyde (MDA). Furthermore, the antioxidant enzyme activities were obviously enhanced. However, the higher concentration of SNP (100 mu mol L-1) enhanced the effects of drought stress for plant. For further analysis of the response mechanism to exogenous NO, the methylation-sensitive amplified polymorphism (MSAP) technique was used to investigate the changes of DNA methylation. When the seedlings of Dendrobium huoshanense were treated with 50 mu mol L-1 SNP containing 10% PEG-6000, levels of global DNA methylation of Dendrobium huoshanense were decreased. Nevertheless, the demethylation rate of methylated sites increased, accounting for 12.5% of total methylation sites. These results implied that some expressed genes were involved in the response process to drought stress triggered by NO in Dendrobium huoshanense.

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