Fine mapping and identification of the soybean R-SC4 resistance candidate gene to soybean mosaic virus

文献类型: 外文期刊

第一作者: Wang, Dagang

作者: Wang, Dagang;Ma, Ying;Liu, Ning;Yang, Zhonglu;Zheng, Guijie;Zhi, Haijian;Wang, Dagang

作者机构:

关键词: soybean;soybean mosaic virus;genomic-SSR fine mapping;resistance genes

期刊名称:PLANT BREEDING ( 影响因子:1.832; 五年影响因子:1.956 )

ISSN: 0179-9541

年卷期: 2011 年 130 卷 6 期

页码:

收录情况: SCI

摘要: Soybean mosaic virus (SMV) disease is one of the most destructive viral diseases for soybeans worldwide. In this study, 1047 F-2 plants derived from 'Dabaima' (resistant) x 'Nannong1138-2' (susceptible) were used to study inheritance and resistance gene linkage to SMV strain SC4. SSR were used to establish genetic maps of the parent plants, in addition to resistant or susceptible F-2 generation plants. Results indicated that a single dominant gene (designated R-SC4) located on chromosome 14 (MLG B2) controls resistance to SC4 infections. In addition, SNP and genomic-SSR markers near R-SC4 were examined. Based on the linkage analysis of the population, the genomic-SSR markers BARCSOYSSR_14_1413 and BAR-CSOYSSR_14_1416 were found flanking R-SC4. Sequence analysis of the soybean genome indicated that the interval between the two genomic-SSR markers was <100 kb on chromosome 14. Quantitative real-time PCR (QRT-PCR) analysis of the 100-kb region identified three genes (Glyma14g38510, 38560 and 38580) likely involved in regulating resistance to SMV. These results are useful for transferring and pyramiding or map-based cloning of R-SC4.

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