DNA extraction from crayfish exoskeleton

文献类型： 外文期刊

第一作者： Li, Yanhe

作者： Li, Yanhe;Wang, Weimin;Liu, Xiaolian;Luo, Wei;Zhang, Jie;Gul, Yasmeen;Li, Yanhe

作者机构：

关键词： Crayfish exoskeleton;DNA amplification;DNA extraction

期刊名称：INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY （ 影响因子：0.818； 五年影响因子：1.006 ）

ISSN： 0019-5189

年卷期： 2011 年 49 卷 12 期

页码：

收录情况： SCI

摘要： Crayfish exoskeleton (CE) samples are generally less invasive and easy to be collected. However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy and efficient DNA extraction protocol for polymerase chain reactions. Specific primer pair (PPO-F, PPO-R) was used to amplify extracted DNA from CE, and compared to crayfish tail muscle DNA sample. Moreover, seven microsatellites markers were used to amplify the CE DNA samples set. Since the extracted DNA from CE is suitable for gene amplification, the results present usefulness of CE as an easy and convenient DNA source for PCR-based population genetic research.

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