Molecular and functional characterization of a cystatin analogue in large yellow croaker (Pseudosciaena crocea)

文献类型： 外文期刊

第一作者： Li, Shuying

作者： Li, Shuying;Ao, Jingqun;Chen, Xinhua;Li, Shuying;Li, Shuying

作者机构：

关键词： Large yellow croaker (Pseudosciaena crocea);Cystatin;Immunomodulatory function;Tumor necrosis factor-alpha;Interleukin-10;Poly(I:C);Inactivated trivalent bacterial vaccine

期刊名称：MOLECULAR IMMUNOLOGY （ 影响因子：4.407； 五年影响因子：4.227 ）

ISSN： 0161-5890

年卷期： 2009 年 46 卷 8-9 期

页码：

收录情况： SCI

摘要： The cDNA of a cystatin analogue was isolated from the spleen Smart cDNA library of large yellow croaker Pseudosciaena crocea (Lyccys). The open reading frame (ORF) of 354 nucleotides (nt) of Lyccys encodes a protein of 118 amino acids (aa), containing a 21-aa signal peptide and a 97-aa mature polypeptide. The deduced Lyccys possessed structural features of the Family II cystatins, including three evolutionally conserved motifs known to interact with the active sites of cysteine peptidases: Gly at the N-terminus (Gly(25)), Gln-X-Val-X-Gly motif (Q(69)LVAG(73)) and Pro-Try pair at the C-terminus (p(106)W(107)). Genomic analysis revealed that Lyccys gene, spanning 2297 nt, consisted of three exons and two introns. The Lyccys gene was constitutively expressed in all eight tissues examined although at different levels. Real-time PCR analysis revealed that Lyccys transcript in spleen and kidney was obviously up-regulated by poly(I:C) or inactivated trivalent bacterial vaccine, while in blood its expression was down-regulated. Immunoelectron microscopy showed that Lyccys was mainly localized to the rough endoplasmic reticulum (rER) or in the vesicular structures in spleen and kidney cells. Recombinant Lyccys protein fused with glutathione S-transferase (rLyccys) was shown to have remarkable protease-inhibitory activity and well affinity binding to papain (with a K(i) of 1.3 x 10(-13) M). An in vivo administration of rLyccys could significantly up-regulate the expression levels of large yellow croaker tumor necrosis factor-alpha 2 (TNF-alpha 2) and interieukin-10 in spleen and kidney, but to a lesser extent increase TNF-alpha 1 expression. These results suggest that the Lyccys is a secreted inhibitor of cysteine proteinases, which may have an immunomodulatory function in inflammation response. (C) 2009 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献

[1]Isolation, modulatory functions on murine B cell development and antigen-specific immune responses of BP11, a novel peptide from the chicken bursa of Fabricius. Liu, Xiao-dong,Feng, Xiu-li,Zhou, Bin,Cao, Rui-Bing,Li, Xin-feng,Chen, Pu-Yan,Ma, Zhi-Yong.

[2]Peroxiredoxin IV Regulates Pro-Inflammatory Responses in Large Yellow Croaker (Pseudosciaena crocea) and Protects against Bacterial Challenge. Yu, Suhong,Mu, Yinnan,Ao, Jingqun,Chen, Xinhua,Yu, Suhong.

[3]Course of infection by Babesia sp BQ1 (Lintan) and B-divergens in sheep depends on the production of IFN gamma and IL10. Moreau, E.,Guan, G.,Yin, H.,Luo, J.. 2010

[4]The integrity of PRRSV nucleocapsid protein is necessary for up-regulation of optimal interleukin-10 through NF-kappa B and p38 MAPK pathways in porcine alveolar macrophages. Liu, Yanyan,Zhang, Yuyu,Zhu, Xiwang,Ren, Sufang,Guo, Lihui,Sun, Wenbo,Chen, Zhi,Cong, Xiaoyan,Chen, Lei,Du, Yijun,Li, Jun,Wu, Jiaqiang,Wang, Jinbao,Yu, Jiang,Liu, Yanyan,Zhang, Yuyu,Zhu, Xiwang,Ren, Sufang,Guo, Lihui,Sun, Wenbo,Chen, Zhi,Cong, Xiaoyan,Chen, Lei,Shi, Jianli,Du, Yijun,Li, Jun,Wu, Jiaqiang,Wang, Jinbao,Liu, Yanyan,Zhu, Xiwang,Wu, Jiaqiang,Wang, Jinbao,Liu, Xing. 2017

[5]Diversity of TNF-alpha region in Chinese domestic goats. E, G. X.,Na, R. S.,Zhao, Y. J.,Huang, Y. F.,Ma, Y. H.. 2015

[6]Dietary black-grained wheat intake improves glycemic control and inflammatory profile in patients with type 2 diabetes: a randomized controlled trial. Liu, Yanping,Qiu, Ju,Yue, Yanfen,Li, Kang,Ren, Guixing. 2018

[7]Effects of spray-dried animal plasma on serous and intestinal redox status and cytokines of neonatal piglets. Gao, Y. Y.,Jiang, Z. Y.,Lin, Y. C.,Zheng, C. T.,Zhou, G. L.,Chen, F.,Gao, Y. Y..

[8]Molecular characterization and expression analysis of MHC class II alpha and beta genes in large yellow croaker (Pseudosciaena crocea). Yu, Suhong,Ao, Jingqun,Chen, Xinhua,Yu, Suhong,Yu, Suhong. 2010

[9]Molecular characterization and expression analysis of beta(2)-microglobulin in large yellow croaker Pseudosciaena crocea. Yu, Suhong,Chen, Xinhua,Ao, Jingqun,Yu, Suhong,Yu, Suhong. 2009

[10]A secreted cystatin from the tick Haemaphysalis longicornis and its distinct expression patterns in relation to innate immunity. Zhou, Jinlin,Ueda, Mami,Umemiya, Rika,Battsetseg, Badgar,Boldbaatar, Damdinsuren,Xuan, Xuenan,Fujisaki, Kozo.

[11]Cloning and prokaryotic expression of Chinese sturgeons cystatin cDNA. Bai, J.,Ma, D.,Lao, H.,Jian, Q.,Ye, X.,Luo, J.. 2005

[12]Functional characterization of a cystatin from the tick Rhipicephalus haemaphysaloides. Wang, Yujian,Zhou, Yongzhi,Gong, Haiyan,Cao, Jie,Zhang, Houshuang,Zhou, Jinlin,Wang, Yujian,Li, Xiangrui,Zhou, Jinlin. 2015

[13]Identification of Ramie Genes in Response to Pratylenchus coffeae Infection Challenge by Digital Gene Expression Analysis. Yu, Yongting,Zeng, Liangbin,Yan, Zhun,Liu, Touming,Sun, Kai,Zhu, Taotao,Zhu, Aiguo. 2015

[14]Characterization of Hlcyst-3 as a member of cystatins from the tick Haemaphysalis longicornis. Zhou, Jinlin,Liao, Min,Fujisaki, Kozo,Zhou, Jinlin,Gong, Haiyan,Xuan, Xuenan,Fujisaki, Kozo. 2010

[15]Search for Nodulation and Nodule Development-Related Cystatin Genes in the Genome of Soybean (Glycine max). Yuan, Songli,Li, Rong,Chen, Haifeng,Zhang, Chanjuan,Chen, Limiao,Hao, Qingnan,Shan, Zhihui,Zhang, Xiaojuan,Chen, Shuilian,Yang, Zhonglu,Qiu, Dezhen,Zhou, Xinan,Yuan, Songli,Li, Rong,Chen, Haifeng,Zhang, Chanjuan,Chen, Limiao,Hao, Qingnan,Shan, Zhihui,Zhang, Xiaojuan,Chen, Shuilian,Yang, Zhonglu,Qiu, Dezhen,Zhou, Xinan,Wang, Lei. 2016

[16]Characterization of a secreted cystatin from the tick Rhipicephalus haemaphysaloides. Wang, Yujian,Yu, Xinmao,Cao, Jie,Zhou, Yongzhi,Gong, Haiyan,Zhang, Houshuang,Zhou, Jinlin,Wang, Yujian,Li, Xiangrui.

[17]Identification and comparative analysis of the Pseudosciaena crocea microRNA transcriptome response to poly(I:C) infection using a deep sequencing approach. Qi, Pengzhi,Guo, Baoying,Zhu, Aiyi,Wu, Changwen,Liu, Changlin.

[18]Immune responses of porcine airway epithelial cells to poly(I:C), a synthetic analogue of viral double-stranded RNA. Yang, Xiu-qin,Li, Hai-tao,Liu, Di,Wang, Liang,Liu, Di.

[19]Poly(I:C) combined with multi-epitope protein vaccine completely protects against virulent foot-and-mouth disease virus challenge in pigs. Cao, Yimei,Lu, Zengjun,Li, Yanli,Sun, Pu,Li, Dong,Li, Pinghua,Bai, Xingwen,Fu, Yuanfang,Bao, Huifang,Zhou, Chunxue,Xie, Baoxia,Chen, Yingli,Liu, Zaixin. 2013

[20]Resiquimod and polyinosinic-polycytidylic acid formulation with aluminum hydroxide as an adjuvant for foot-and-mouth disease vaccine. Zhou, Chun-Xue,Li, Dong,Chen, Ying-Li,Lu, Zeng-Jun,Sun, Pu,Cao, Yi-Mei,Bao, Hui-Fang,Fu, Yuan-Fang,Li, Ping-Hua,Bai, Xing-Wen,Xie, Bao-Xia,Liu, Zai-Xin. 2014

作者其他论文 更多>>

微信小程序