文献类型： 外文期刊

第一作者： Liu, Yufang

作者： Liu, Yufang;Guo, Siwu;He, Xiaoyun;Chu, Mingxing;Jiang, Yanting;Hong, Qionghua;Lan, Rong

作者机构：

关键词： goat; fertility; RBP4; TFDP1; granulosa cell proliferation

期刊名称：CELLS （ 影响因子：7.666； 五年影响因子：7.677 ）

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年卷期： 2022 年 11 卷 14 期

页码：

收录情况： SCI

摘要： Retinol-binding protein 4 (RBP4), a member of the lipocalin family, is a specific carrier of retinol (vitamin A) in the blood. Numerous studies have shown that RBP4 plays an important role in mammalian embryonic development and that mutations in RBP4 can be used for the marker-assisted selection of animal reproductive traits. However, there are few studies on the regulation of reproduction and high-prolificacy traits by RBP4 in goats. In this study, the 5 ' flanking sequence of RBP4 was amplified, and a G>C polymorphism in the promoter region -211 bp (g.36491960) was detected. An association analysis revealed that the respective first, second and third kidding number and mean kidding number of nanny goats with CC and GC genotypes (2.167 +/- 0.085, 2.341 +/- 0.104, 2.529 +/- 0.107 and 2.189 +/- 0.070 for CC and 2.052 +/- 0.047, 2.206 +/- 0.057, 2.341 +/- 0.056 and 2.160 +/- 0.039 for GC) were significantly higher (p < 0.05) than those with the GG genotype (1.893 +/- 0.051, 2.027 +/- 0.064, 2.107 +/- 0.061 and 1.74 +/- 0.05). The luciferase assay showed that luciferase activity was increased in C allele individuals compared with that in G allele individuals. A competitive electrophoretic mobility shift assay (EMSA) showed that individuals with the CC genotype had a stronger promoter region binding capacity than those with the GG genotype. In addition, transcription factor prediction software showed that the RBP4 g.36491960G>C mutation added a novel binding site for transcription factor DP-1 (TFDP1). RT-qPCR results showed that the expression of TFDP1 was significantly higher in the high-prolificacy group than in the low-prolificacy group, and the expression of RBP4 was higher in both the CC and GC genotypes than that in the GG genotype. TFDP1 overexpression significantly increased the expression of RBP4 mRNA (p < 0.05) and the expression of the cell proliferation factors cyclin-D1, cyclin-D2 and CDK4 (p < 0.05). The opposite trend was observed after interference with TFDP1. Both the EdU and CCK-8 results showed that TFDP1 expression could regulate the proliferation of goat ovarian granulosa cells. In summary, our results showed that RBP4 g.36491960G>C was significantly associated with fecundity traits in goats. The g.36491960G>C mutation enhanced the transcriptional activity of RBP4 and increased the expression of RBP4, thus improving the fertility of Yunshang black goats.

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