Characterization of the genome structure of Bombyx mori densovirus (China isolate)

文献类型： 外文期刊

第一作者： Wang, Yong Jie

作者： Wang, Yong Jie;Yao, Qin;Chen, Ke Ping;Wang, Yong;Lu, Jian;Han, Xu

作者机构：

关键词： BmDNV-3;genome structure;sequence analysis;transcription strategy

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN： 0920-8569

年卷期： 2007 年 35 卷 1 期

页码：

收录情况： SCI

摘要： The genome of Bombyx mori densovirus (China isolate), termed as BmDNV-3, is composed of two kinds of different single-stranded linear DNA molecules (VD1 and VD2). In this study, the viral DNA molecules were purified and cloned into pUC119 vector, and the complete nucleotide sequence was determined. Sequence analysis showed that VD1 genome consisted of 6,543 nts including inverted terminal repeats (ITRs) of 224 nts, and VD2 genome consisted of 6,022 nts including ITRs of 524 nts. Comparison of the complete genome sequence between BmDNV-3 and BmDNV-2 (Yamanashi isolate) showed an identity of 98.4% in VD1 and 97.7% in VD2, with a total number of 228 bp substitutions, 11 bp deletions and 3 bp insertions found in BmDNV-3. A single nucleotide "A" deletion at nt 1589 in BmDNV-3 caused a frame shift mutation and brought about a premature stop codon, thus dividing VD2 of BmDNV-3 into two ORFs (named VD2 ORF1a and VD2 ORF1b) within that region, while there was only one ORF (named VD2 ORF1) in the corresponding region of BmDNV-2 (Yamanashi isolate). Comparative polymorphisms of ORFs and ITR regions of the two viral genomes showed that highly variable regions were mainly located in VD1 ORF3, VD1 ORF4, VD2 ORF2, and ITRs of BmDNV-3. Northern blots analysis revealed that VD1 had 1.1 kb and 1.5 kb transcripts from the left half of its plus strand, and one transcript about 3.3 kb from the right half of its minus strand. Sequencing of 3' and 5' RACE products showed that the 1.1 kb transcript started at nt 290 and ended at nt 1437, the 1.5 kb transcript started at nt 1423 and ended at nt 2931, and the 3.3 kb transcript started at nt 6287 and ended at nt 2922. These results help us to further understand the variation between different DNV genera and its possible causes, providing clues for studying the evolutionary history of densoviruses.

分类号：

  • 相关文献

[1]The complete mitochondrial genome of Eleotris oxycephala (Perciformes: Eleotridae). Meng, Yongyong,Ma, Hongyu,Ma, Chunyan,Wei, Hongqing,Liu, Yuexing,Zhang, Fengying,Wang, Wei,Chen, Wei,Zhao, Mengdi,Chen, Fenfang,Ma, Lingbo,Meng, Yongyong,Wei, Hongqing,Liu, Yuexing,Zhao, Mengdi,Chen, Fenfang. 2016

[2]The complete mitochondrial genome sequence and gene organization of Trematomus bernacchii (Perciformes: Nototheniidae) with phylogenetic consideration. Song, Wei,Li, Lingzhi,Huang, Hongliang,Zhao, Mengdi,Jiang, Keji,Zhang, Fengying,Zhao, Ming,Chen, Xuezhong,Ma, Lingbo,Zhao, Mengdi. 2016

[3]The complete mitochondrial genome sequence and gene organization of Tridentiger trigonocephalus (Gobiidae: Gobionellinae) with phylogenetic consideration. Wei, Hongqing,Ma, Hongyu,Ma, Chunyan,Zhang, Fengying,Wang, Wei,Chen, Wei,Ma, Lingbo,Wei, Hongqing.

[4]The complete mitochondrial genome of Chionodraco hamatus (Notothenioidei: Channichthyidae) with phylogenetic consideration. Song, Wei,Li, Lingzhi,Huang, Hongliang,Meng, Yongyong,Jiang, Keji,Zhang, Fengying,Chen, Xuezhong,Ma, Lingbo. 2016

[5]The complete mitochondrial genome of southern flounder Paralichthys lethostigma (Pleuronectiformes, Bothidae). Shi, Bao,Liu, Xuezhou,Xu, Yongjiang,Wang, Bin,Shi, Bao,Liu, Xuezhou,Xu, Yongjiang,Wang, Bin. 2016

[6]Differential genome evolution and speciation of Coix lacryma-jobi L. and Coix aquatica Roxb. hybrid guangxi revealed by repetitive sequence analysis and fine karyotyping. Cai, Zexi,Liu, Huijun,He, Qunyan,Pu, Mingwei,Jin, Weiwei,He, Qunyan,Chen, Jian,Lai, Jinsheng,Chen, Jian,Lai, Jinsheng,Li, Xuexian. 2014

[7]The complete mitochondrial genome of the Sichuan taimen (Hucho bleekeri): Repetitive sequences in the control region and phylogenetic implications for Salmonidae. Du, Jun,Wang, Ying,Guo, Rui,Zhang, Xiuyue,Song, Zhaobin.

[8]Genetic linkage maps of barfin flounder (Verasper moseri) and spotted halibut (Verasper variegatus) based on AFLP and microsatellite markers. Ma, Hongyu,Chen, Songlin,Yang, Jingfeng,Chen, Siqing,Ma, Hongyu,Liu, Hanwen.

[9]Gene networks in the synthesis and deposition of protein polymers during grain development of wheat. She, Maoyun,Ye, Xingguo,Belgard, M.,Ma, Wujun,Belgard, M.,Ma, Wujun,Yan, Yueming,Howit, C..

[10]Complete genomic sequence analyses of Turnip mosaic virus basal-BR isolates from China. Wang, Hong-Yan,Liu, Jin-Liang,Gao, Rui,Chen, Jia,Shao, Yun-Hua,Li, Xiang-Dong,Wang, Hong-Yan,Liu, Jin-Liang,Gao, Rui. 2009

[11]Cloning and Characterization of Phospholipases A2 and Hyaluronidase Genes from the Venom of the Honeybee Apis mellifera carnica (Hymenoptera: Apidae). Hou, Chunsheng,Guo, Liqiong,Wang, Jianrong,You, Linfeng,Lin, Junfang,Wu, Wuhua,Wu, Chengshun,Wang, Teng,Hou, Chunsheng,Guo, Liqiong,Wang, Jianrong,You, Linfeng,Lin, Junfang,Wu, Wuhua,Wu, Chengshun,Wang, Teng,Hou, Chunsheng.

[12]Differential gene expression of cotton cultivar CCRI24 during somatic embryogenesis. Xiuming Wu,Fuguang Li#2;,Chaojun Zhang,Chuanliang Liu,Xueyan Zhang.

[13]Molecular cloning, genomic structure and expression analysis of major histocompatibility complex class I alpha gene of half-smooth tongue sole (Cynoglossus semilaevis). Chen, Song-lin,Xu, Tian-jun. 2011

[14]Cloning and Characterization of Goose PPAR gamma Gene Comparison with Other Vertebrates. Wu, H. L.,Kong, F. L.,Qiu, M. H.,Zhou, B.,Luo, J.,Zhu, Q.,Wang, H. Y.,Liu, Y.,He, D. Q.. 2012

[15]Sequence analysis of the haemagglutinin genes of H9N2 avian influenza viruses isolated in China during 1996 to 2011. Dai, Yin,Zhao, Ruihong,Hu, Xiaomiao,Zhang, Danjun,Wang, Junjun,Xu, Fazhi. 2012

[16]Analysis of the gene 3 region sequences of Chinese field strains of Transmissible gastroenteritis virus. Wang, C. B.,Feng, L.,Chen, J. F.,Shi, H. Y.,Qiu, H. J.,Xue, F.,Zhu, Y. M.,Liu, S. W.. 2010

[17]Molecular characterization and expression pattern of the porcine STARS, a striated muscle-specific expressed gene. Peng, Y. B.,Guan, H. P.,Fan, B.,Zhao, S. H.,Xu, X. W.,Li, K.,Zhu, M. J.,Liu, B.,Li, K.,Yerle, M.. 2008

[18]Analysis of S1 gene of avian infectious bronchitis virus isolated in southern China during 2011-2012. Feng, Keyu,Chen, Feng,Xie, Qingmei,Feng, Keyu,Xie, Qingmei,Xue, Yu,Wang, Feng,Shu, Dingming,Xie, Qingmei. 2014

[19]First report of the melon stem rot disease in protected cultivation caused by Pseudomonas fluorescens. Zhang, Chenghua,Wang, Yiwen,Zhu, Pinkuan,Xu, Ling,Lin, Tao,Li, Jufen,Ma, Guobin.

[20]Attenuation of an original US porcine epidemic diarrhea virus strain PC22A via serial cell culture passage. Lin, Chun-Ming,Hou, Yixuan,Gao, Xiang,Liu, Xinsheng,Zheng, Lanlan,Saif, Linda J.,Wang, Qiuhong,Marthaler, Douglas G.,Marthaler, Douglas G.,Liu, Xinsheng,Zheng, Lanlan.

作者其他论文 更多>>

微信小程序