Comparison of base substitutions in response to nitrogen ion implantation and Co-60-gamma ray irradiation in Escherichia coli

文献类型： 外文期刊

第一作者： Xie, CX

作者： Xie, CX;Xu, A;Wu, LJ;Yao, JM;Yang, JB;Yu, ZL

作者机构：

关键词： base substitution;low-energy nitrogen ion beam implantation;Co-60-gamma rays;Escherichia coli

期刊名称：GENETICS AND MOLECULAR BIOLOGY （ 影响因子：1.771； 五年影响因子：2.584 ）

ISSN： 1415-4757

年卷期： 2004 年 27 卷 2 期

页码：

收录情况： SCI

摘要： To identify the specificity of base substitutions, a novel experimental system was established based on rifampicin-resistant (Rif') mutant screening and sequencing of the defined region of the rpoB gene in E coli. We focused on comparing mutational spectra of base substitutions induced by either low energy nitrogen ion beam implantation or Co-60-gamma rays. The most significant difference in the frequency of specific kinds of mutations induced by low energy nitrogen ion beam was that CG --> TA transitions were significantly increased from 32 to 46, AT --> TA transversions were doubled from 7 to 15 in 50 mutants, respectively. The preferential base substitutions induced by nitrogen ion beam implantation were CG --> TA transitions, AT --> GC transitions, AT --> TA transversions, which account for 92.13% (82/89) of the total. The mutations induced by Co-60-gamma rays were preferentially GC --> AT and AT --> GC transitions, which totaled 84.31% (43/51).

分类号：

  • 相关文献

[1]Effect of enrofloxacin on gene expression profiles of Escherichia coli. Bai, Hua,Su, Wen-zheng,Zhu, Xiao-ling,Hu, Ming,Liu, Yu-qing,Bai, Hua,Su, Wen-zheng,Zhu, Xiao-ling,Hu, Ming,Liu, Yu-qing.

[2]Porcine parvovirus capsid protein expressed in Escherichia coli self assembles into virus-like particles with high immunogenicity in mice and guinea pigs. Ji, Pengchao,Zhou, Enmin,Ji, Pengchao,Liu, Yunchao,Chen, Yumei,Jiang, Dawei,Zhao, Baolei,Wang, Jvcai,Chai, Shujun,Zhang, Gaiping,Wang, Aiping,Zhang, Gaiping,Jiang, Dawei,Zhao, Baolei,Wang, Jvcai,Zhang, Gaiping,Zhang, Gaiping.

[3]Putative carotenoid genes expressed under the regulation of Shine-Dalgarno regions in Escherichia coli for efficient lycopene production. Jin, Weiyue,Xu, Xian,Li, Shuang,Huang, He,Jiang, Ling,Zhang, Zhidong,Huang, He.

[4]Cloning, expression and characterization of a trehalose synthase gene from rhodococcus opacus. Yan, Junyan,Qiao, Yu,Ding, Hongbiao,Hu, Jun,Ding, Hongbiao.

[5]Cloning, expression and characterization of Kunming mice lactoferrin and its N-lobe. Tian, Zigang,Teng, Da,Yang, Yalin,Wang, Jianhua,Wang, Jiarong,Tian, Zigang,Teng, Da,Yang, Yalin,Wang, Jianhua,Wang, Jiarong,Hu, Jiancheng.

[6]Relationship between differential retention of Escherichia coli and Enterococcus faecalis and variations in enzyme activity in the scallop Patinopecten yessoensis. Li, Bin,Chen, Bijuan,Jiang, Zengjie,Zhang, Jihong,Fang, Jianguang,Li, Bin,Li, Bin,Qi, Zhanhui.

[7]Expression of antimicrobial peptide LH multimers in Escherichia coli C43(DE3). Tian, Zi-gang,Dong, Tian-tang,Yang, Ya-lin,Teng, Da,Wang, Jian-hua.

[8]Multimerization and fusion expression of bovine lactoferricin derivative LfcinB15-W4,10 in Escherichia coli. Tian, Zi-gang,Da Teng,Yang, Ya-lin,Luo, Jin,Feng, Xing-jun,Fan, Ying,Zhang, Fan,Wang, Jian-hua.

[9]Isolation, phylogenetic group, drug resistance, biofilm formation, and adherence genes of Escherichia coli from poultry in central China. Wang, Yang,Wang, Yuxin,Zhao, Wenpeng,Yi, Li,Wang, Yang,Ding, Chan,Wang, Yang,Wang, Yuanguo,Cai, Ying.

[10]MRT letter: Localization of endogenous hydrogen peroxide by modified processes of sample preparation for transmissionelectron microscope in Escherichia coli. Hu, Rongliu,Zhu, Wenxue,Fan, Jinling,Wang, Na,Wang, Liping,Yang, Lipeng,Li, Xin,He, Chenyang,Pang, Xinyue,Zhao, Chunyan.

[11]Inactivation of Escherichia coli O157:H7 in vitro and on the surface of spinach leaves by biobased antimicrobial surfactants. Zhang, Xuejie,Yan, Ruixiang,Zhang, Xuejie,Fan, Xuetong,Solaiman, Daniel K. Y.,Ashby, Richard D.,Mukhopadhyay, Sudarsan,Liu, Zengshe,Yan, Ruixiang.

[12]Global regulator engineering significantly improved Escherichia coli tolerances toward inhibitors of lignocellulosic hydrolysates. Wang, Jianqing,Zhang, Yan,Lin, Zhanglin,Chen, Yilu,Lin, Min.

[13]Growth performance, gastrointestinal microbial activity, and immunological response of piglets receiving microencapsulated Enterococcus faecalis CG1.0007 and enzyme complex after an oral challenge with Escherichia coli (K88). Chen, H. S.,Feng, Z.,Liu, D.,Chen, H. S.,Velayudhan, D. E.,Nyachoti, C. M.,Li, A.,Yin, Y. L..

[14]Presence and Antimicrobial Resistance of Escherichia coli in Ready-to-Eat Foods in Shaanxi, China. Baloch, Allah Bux,Feng, Yuqing,Xi, Meili,Wu, Qian,Yang, Qinhao,Tang, Jingsi,He, Xiangxiang,Xia, Xiaodong,Xia, Xiaodong,Yang, Hua,Xiao, Yingping,Yang, Hua,Xiao, Yingping.

[15]Enhanced tolerance and accumulation of heavy metal ions by engineered Escherichia coli expressing Pyrus calleryana phytochelatin synthase. Li, Hui,Lin, Jing,Chang, Youhong,Cong, Yu.

[16]Morphology analysis of Escherichia coli treated with nonthermal plasma. Guo, J.,Li, Z.,Huang, K.,Li, Y.,Wang, J.,Guo, J.,Li, Y..

[17]Dual effectiveness of sodium chlorite for enzymatic browning inhibition and microbial inactivation on fresh-cut apples. Luo, Yaguang,Lu, Shengmin,Zhou, Bin,Lu, Shengmin,Zhou, Bin,Feng, Hao. 2011

[18]Polysialic acid biosynthesis and production in Escherichia coli: current state and perspectives. Lin, Bai-Xue,Tao, Yong,Qiao, Yu,Shi, Bo.

[19]Recombinant expressions of sweet plant protein mabinlin II in Escherichia coli and food-grade Lactococcus lactis. Gu, Wenliang,Xia, Qiyu,Fu, Shaoping,Guo, Jianchun,Yao, Jing,Hu, Xinwen.

[20]High-Yield Recombinant Expression of the Chicken Antimicrobial Peptide Fowlicidin-2 in Escherichia coli. Feng, Xingjun,Xu, Wenshan,Qu, Pei,Li, Xiaochong,Xing, Liwei,Liu, Di,Jiao, Jian,Wang, Jue,Li, Zhongqiu,Liu, Chunlong,Liu, Chunlong.

作者其他论文 更多>>

微信小程序