Comparison of base substitutions in response to nitrogen ion implantation and Co-60-gamma ray irradiation in Escherichia coli

文献类型： 外文期刊

第一作者： Xie, CX

作者： Xie, CX;Xu, A;Wu, LJ;Yao, JM;Yang, JB;Yu, ZL

作者机构：

关键词： base substitution;low-energy nitrogen ion beam implantation;Co-60-gamma rays;Escherichia coli

期刊名称：GENETICS AND MOLECULAR BIOLOGY （ 影响因子：1.771； 五年影响因子：2.584 ）

ISSN： 1415-4757

年卷期： 2004 年 27 卷 2 期

页码：

收录情况： SCI

摘要： To identify the specificity of base substitutions, a novel experimental system was established based on rifampicin-resistant (Rif') mutant screening and sequencing of the defined region of the rpoB gene in E coli. We focused on comparing mutational spectra of base substitutions induced by either low energy nitrogen ion beam implantation or Co-60-gamma rays. The most significant difference in the frequency of specific kinds of mutations induced by low energy nitrogen ion beam was that CG --> TA transitions were significantly increased from 32 to 46, AT --> TA transversions were doubled from 7 to 15 in 50 mutants, respectively. The preferential base substitutions induced by nitrogen ion beam implantation were CG --> TA transitions, AT --> GC transitions, AT --> TA transversions, which account for 92.13% (82/89) of the total. The mutations induced by Co-60-gamma rays were preferentially GC --> AT and AT --> GC transitions, which totaled 84.31% (43/51).

分类号：

  • 相关文献

[1]Antimicrobial resistance in Escherichia coli isolates from food animals, animal food products and companion animals in China. Lei, Tao,Tian, Wei,He, Liu,Huang, Xian-Hui,Sun, Yong-Xue,Deng, Yu-Ting,Sun, Yan,Liu, Jian-Hua,Lv, Dian-Hong,Wu, Cong-Ming,Shen, Jian-Zhong,Huang, Liang-Zong. 2010

[2]High prevalence and widespread distribution of multi-resistant Escherichia coli isolates in pigs and poultry in China. Jiang, Hong-Xia,Chen, Zhang-Liu,Wang, Xiu-Mei,Chen, Ji-Rong,Liu, Ya-Hong,Liao, Xiao-Ping,Liu, Jian-Hua,Zeng, Zhen-Ling,Lue, Dian-Hong. 2011

[3]Paving the way to construct a new vaccine against Escherichia coli from its recombinant outer membrane protein C via a murine model. Wang, Xiao,Guan, Qingfeng,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Wang, Jianhua,Wang, Xiao,Yao, Junhu,Wang, Xiao,Guan, Qingfeng,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Wang, Jianhua. 2015

[4]Fitness Advantage of mcr-1-Bearing IncI2 and IncX4 Plasmids in Vitro. Wu, Renjie,Yi, Ling-xian,Yu, Lin-feng,Wang, Jing,Liu, Yiyun,Chen, Xiaojie,Lv, Luchao,Yang, Jun,Liu, Jian-Hua,Chen, Xiaojie. 2018

[5]Evaluation of Sanitizing Methods for Reducing Microbial Contamination on Fresh Strawberry, Cherry Tomato, and Red Bayberry. Wei, Wei,Wang, Xu,Xie, Zhongwen,Zhou, Yu,Wei, Wei,Wang, Wen,Xu, Junfeng,Liu, Yuanjing,Gao, Haiyan. 2017

[6]Cloning, expression and characterization of a cold-adapted endo-1, 4-beta-glucanase from Citrobacter farmeri A1, a symbiotic bacterium of Reticulitermes labralis. Bai, Xi,Yuan, Xianjun,Li, Junfeng,Shao, Tao,Wen, Aiyou,Bai, Yunfeng. 2016

[7]An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species. Gomaa, Ahmed E.,Zhang, Chen,Yang, Zhimin,Shang, Liguo,Jiang, Shijie,Deng, Zhiping,Zhan, Yuhua,Lu, Wei,Lin, Min,Yan, Yongliang. 2017

[8]High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway. Fang, Ming-Yue,Zhang, Chong,Xing, Xin-Hui,Yang, Song,Cui, Jin-Yu,Jiang, Pei-Xia,Lou, Kai,Wachi, Masaaki. 2015

[9]Inhibition of Citrate Synthase Thermal Aggregation In Vitro by Recombinant Small Heat Shock Proteins. Wan, Fanghao,Guo, Jianying,Gong Weina,Xie, Bingyan,Yue, Ming. 2009

[10]Molecular epidemiology of colistin-resistant Enterobacteriaceae in inpatient and avian isolates from China: high prevalence of mcr-negative Klebsiella pneumoniae. Wang, Xiaojuan,Wang, Ruobing,Jin, Longyang,Zhang, Yawei,Wang, Qi,Chen, Hongbin,Wang, Hui,Liu, Yuqing,Zhao, Min,Qi, Xiaomei,Qi, Xiaomei,Qi, Xiaomei. 2017

[11]Improvement of an E. coli bioreporter for monitoring trace amounts of phenol by deletion of the inducible sigma(54)-dependent promoter. Peng, Zixin,Yan, Yongliang,Xu, Yuquan,Yu, Haiying,Zhan, Yuhua,Zhang, Wei,Lin, Min,Chen, Ming,Takeo, Masahiro,Zhao, Zhonglin. 2010

[12]Solution for promoting egl3 gene of Trichoderma reesei high-efficiency secretory expression in Escherichia coli and Lactococcus lactis. Liu, Qinhua,Shao, Tao,Dong, Zhihao,Bai, Yunfeng. 2017

[13]Curing a large endogenous plasmid by single substitution of a partitioning gene. Song, Ningning,Li, Zhaoli,Li, Zhaoli,Hernalsteens, Jean-Pierre,Song, Ningning,Xu, Jie.

[14]Catalytic efficiency of HAP phytases is determined by a key residue in close proximity to the active site. Fu, Dawei,Li, Zhongyuan,Huang, Huoqing,Yuan, Tiezheng,Shi, Pengjun,Luo, Huiying,Meng, Kun,Yang, Peilong,Yao, Bin.

[15]Cloning, expression and characterization of Kunming mice lactoferrin and its N-lobe. Tian, Zigang,Teng, Da,Yang, Yalin,Wang, Jianhua,Wang, Jiarong,Tian, Zigang,Teng, Da,Yang, Yalin,Wang, Jianhua,Wang, Jiarong,Hu, Jiancheng.

[16]Global regulator engineering significantly improved Escherichia coli tolerances toward inhibitors of lignocellulosic hydrolysates. Wang, Jianqing,Zhang, Yan,Lin, Zhanglin,Chen, Yilu,Lin, Min.

[17]Relationship between differential retention of Escherichia coli and Enterococcus faecalis and variations in enzyme activity in the scallop Patinopecten yessoensis. Li, Bin,Chen, Bijuan,Jiang, Zengjie,Zhang, Jihong,Fang, Jianguang,Li, Bin,Li, Bin,Qi, Zhanhui.

[18]Isolation, phylogenetic group, drug resistance, biofilm formation, and adherence genes of Escherichia coli from poultry in central China. Wang, Yang,Wang, Yuxin,Zhao, Wenpeng,Yi, Li,Wang, Yang,Ding, Chan,Wang, Yang,Wang, Yuanguo,Cai, Ying.

[19]Dual effectiveness of sodium chlorite for enzymatic browning inhibition and microbial inactivation on fresh-cut apples. Luo, Yaguang,Lu, Shengmin,Zhou, Bin,Lu, Shengmin,Zhou, Bin,Feng, Hao. 2011

[20]Polysialic acid biosynthesis and production in Escherichia coli: current state and perspectives. Lin, Bai-Xue,Tao, Yong,Qiao, Yu,Shi, Bo.

作者其他论文 更多>>

微信小程序