Resveratrol induces apoptosis and inhibits adipogenesis by stimulating the SIRT1-AMPK alpha-FOXO1 signalling pathway in bovine intramuscular adipocytes

文献类型: 外文期刊

第一作者: Liu, Xiaomu

作者: Liu, Xiaomu;Zhao, Hongbo;Jin, Qing;You, Wei;Cheng, Haijian;Liu, Yifan;Song, Enliang;Liu, Guifen;Tan, Xiuwen;Zhang, Xianglun;Wan, Fachun;Liu, Xiaomu;Zhao, Hongbo;Jin, Qing;You, Wei;Cheng, Haijian;Liu, Yifan;Song, Enliang;Liu, Guifen;Tan, Xiuwen;Zhang, Xianglun;Wan, Fachun

作者机构:

关键词: Resveratrol;Bovine intramuscular adipocytes;Apoptosis;SIRT1;AMPK alpha;FOXO1

期刊名称:MOLECULAR AND CELLULAR BIOCHEMISTRY ( 影响因子:3.396; 五年影响因子:3.282 )

ISSN: 0300-8177

年卷期: 2018 年 439 卷 1-2 期

页码:

收录情况: SCI

摘要: Sirtuin type 1 (SIRTl) and AMP-activated protein kinase (AMPK) play important roles in regulating energy metabolism, cell proliferation and differentiation, ageing, apoptosis, and metabolism. The effect of 100, 200, and 400 mu m Resveratrol (RES), an activator of SIRT1, on apoptosis of bovine intramuscular adipocytes was investigated by nuclear staining, flow cytometry, quantitative real-time polymerase chain reaction, and western blotting. Results show that RES inhibited adipogenesis, decreased cell viability, and increased apoptotic rates in a dose-dependent way. RES up-regulated SIRT1, AMPK alpha, forkhead box O1 (FOXO1), hormone-sensitive lipase (HSL), lipoprotein lipase (LPL), caspase-3, and Bax; and down-regulated peroxisome proliferator-activated receptor-gamma (PPAR gamma), fatty acid synthase (FAS), and Bcl-2, at both mRNA and protein level. The effect of RES was abolished by addition of sirtinol (an inhibitor of SIRT1). This is the first study demonstrating a role for AMPK-SIRT1-FOXO1 signalling pathway in regulating apoptosis in bovine intramuscular adipocytes. Our findings provide important information on the mechanism by which RES controls deposition of cattle intramuscular fat via adipocyte apoptosis.

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