Identification and Expression Analysis of Wheat TaGF14 Genes

文献类型： 外文期刊

第一作者： Guo, Jun

作者： Guo, Jun;Li, Haosheng;Liu, Aifeng;Liu, Cheng;Cheng, Dungong;Cao, Xinyou;Chu, Xiusheng;Zhai, Shengnan;Liu, Jianjun;Zhao, Zhendong;Song, Jianmin;Dai, Shuang

作者机构：

关键词： Triticum aestivum;14-3-3;phylogenetic analysis;tissue-specific expression;starch biosynthesis

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

ISSN： 1664-8021

年卷期： 2018 年 9 卷

页码：

收录情况： SCI

摘要： The 14-3-3 gene family members play key roles in various cellular processes. However, little is known about the numbers and roles of 14-3-3 genes in wheat. The aims of this study were to identify TaGF14 numbers in wheat by searching its whole genome through blast, to study the phylogenetic relationships with other plant species and to discuss the functions of TaGF14s. The results showed that common wheat harbored 20 TaGF14 genes, located on wheat chromosome groups 2, 3, 4, and 7. Out of them, eighteen TaGF14s are non-epsilon proteins, and two wheat TaGF14 genes, TaGF14i and TaGF14f, are epsilon proteins. Phylogenetic analysis indicated that these genes were divided into six clusters: cluster 1 (TaGF14d, TaGF14g, TaGF14j, TaGF14h, TaGF14c, and TaGF14n); cluster 2 (TaGF14k); cluster 3 (TaGF14b, TaGF14l, TaGF14m, and TaGF14s); cluster 4 (TaGF14a, TaGF14e, and TaGF14r); cluster 5 (TaGF14i and TaGF14f); and cluster 6 (TaGF14o, TaGF14p, TaGF14q, and TaGF14t). Tissue-specific gene expressions suggested that all TaGF14s were likely constitutively expressed, except two genes, i.e., TaGF14p and TaGF14f. And the highest amount of TaGF14 transcripts were observed in developing grains at 20 days post anthesis (DPA), especially for TaGF14j and TaGF14l. After drought stress, five genes, i.e., TaGF14c, TaGF14d, TaGF14g, TaGF14h, and TaGF14j, were up-regulated expression under drought stress for both 1 and 6 h, suggesting these genes played vital role in combating against drought stress. However, all the TaGF14s were down-regulated expression under heat stress for both 1 and 6 h, indicating TaGF14s may be negatively associated with heat stress by reducing the expression to combat heat stress or through other pathways. These results suggested that cluster 1, e.g., TaGF14j, may participate in the whole wheat developing stages, e.g., grain-filling (starch biosynthesis) and may also participate in combating against drought stress. Subsequently, a homolog of TaGF14j, TaGF14-JM22, were cloned by RACE and used to validate its function. Immunoblotting results showed that TaGF14-JM22 protein, closely related to TaGF14d, TaGF14g, and TaGF14j, can interact with AGP-L, SSI, SSII, SBEIIa, and SBEIIb in developing grains, suggesting that TaGF14s located on group 4 may be involved in starch biosynthesis. Therefore, it is possible to develop starch-rich wheat cultivars by modifying TaGF14s.

分类号：

  • 相关文献

[1]Molecular Analysis and Expression Patterns of Four 14-3-3 Genes from Brassica napus L.. Zhan Gao-miao,Tong Jin,Wang Han-zhong,Hua Wei. 2010

[2]Evaluation of protective immune responses induced by DNA vaccines encoding Toxoplasma gondii surface antigen 1 (SAG1) and 14-3-3 protein in BALB/c mice. Meng, Min,He, Shenyi,Zhao, Guanghui,Bai, Yang,Zhou, Huaiyu,Cong, Hua,Lu, Gang,Zhao, Qunli,Zhu, Xing-Quan,Meng, Min,He, Shenyi,Zhu, Xing-Quan,Zhu, Xing-Quan. 2012

[3]Accumulation and tolerance to cadmium heavy metal ions and induction of 14-3-3 gene expression in response to cadmium exposure in Coprinus atramentarius. Xie, Chengjian,Yang, Xingyong,Hu, Liujie,Liao, Dunxiu,Yang, Yongzhu.

[4]Molecular cloning and functional analysis of NAC family genes associated with leaf senescence and stresses in Gossypium hirsutum L.. Shah, Syed Tariq,Pang, Chaoyou,Hussain, Anwar,Fan, Shuli,Song, Meizhen,Zamir, Roshan,Yu, Shuxun. 2014

[5]Marker-free, tissue-specific expression of Cry1Ab as a safe transgenic strategy for insect resistance in rice plants. Qi, Yongbin,Chen, Lei,Jin, Qingsheng,Zhang, Xiaoming,Qi, Yongbin,He, Zuhua,He, Xiuling,He, Zuhua. 2013

[6]Genome-Wide Discovery of Tissue-Specific Genes in Maize. Lin, Feng,Bao, Huabin,Zhao, Han,Bao, Huabin,Yang, Jun,Liu, Yuhe,Dai, Huixue.

[7]Highly interactive nature of flower-specific enhancers and promoters, and its potential impact on tissue-specific expression and engineering of multiple genes or agronomic traits. Wen, Zhifeng,Yang, Yazhou,Zhang, Jinjin,Wang, Xiping,Yang, Yingjun,Yan, Guohua,Liu, Zongrang,Wen, Zhifeng,Yang, Yazhou,Wang, Xiping,Zhang, Jinjin,Singer, Stacy,Liu, Zhongchi,Yang, Yingjun,Yan, Guohua.

[8]Increasing maize seed weight by enhancing the cytoplasmic ADP-glucose pyrophosphorylase activity in transgenic maize plants. Wang, Zhangying,Chen, Xiaoping,Wang, Jianhua,Liu, Tingsong,Liu, Yan,Zhao, Li,Wang, Guoying. 2007

[9]A splice site mutation in shrunken1-m causes the shrunken 1 mutant phenotype in maize. Guan, Haiying,He, Chunmei,Liu, Chunxiao,Liu, Qiang,Dong, Rui,Liu, Tieshan,Wang, Liming,Dong, Yongbin,Li, Yuling,Liu, Chaoxian.

[10]MeSAUR1, Encoded by a Small Auxin-Up RNA Gene, Acts as a Transcription Regulator to Positively Regulate ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in Cassava. Ma, Ping'an,Liu, Chen,Meng, Yuhong,Ma, Ping'an,Chen, Xin,Liu, Chen,Meng, Yuhong,Xia, Zhiqiang,Zeng, Changying,Lu, Cheng,Wang, Wenquan,Ma, Ping'an,Chen, Xin,Liu, Chen,Meng, Yuhong,Xia, Zhiqiang,Zeng, Changying,Lu, Cheng,Wang, Wenquan. 2017

[11]OsbZIP58, a basic leucine zipper transcription factor, regulates starch biosynthesis in rice endosperm. Wang, Jie-Chen,Cai, Xiu-Ling,Xu, Heng,Zhu, Ying,Liu, Qiao-Quan. 2013

[12]Du1, encoding a novel Prp1 protein, regulates starch biosynthesis through affecting the splicing of Wx(b) supercript stop pre-mRNAs in rice (Oryza sativa L.). Zeng, Dali,Yan, Meixian,Wang, Yonghong,Liu, Xinfang,Qian, Qian,Li, Jiayang.

[13]Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing. Wu SuoWei,Zheng Xu,Liu BingHua,Yang Li,Song MeiFang,Zhou Peng,Zhou Yang,Meng FanHua,Wang ShanHong,Liu HongWei,Zhai HuQu,Yang JianPing,Xiao Yang,Zheng Xu,Cai YingFan,Yang JianPing,Dolezel, Jaroslav,Song MeiFang. 2010

[14]Characterization of a cell wall invertase gene TaCwi-A1 on common wheat chromosome 2A and development of functional markers. Dongyun Ma,Jun Yan,Zhonghu He,Ling Wu,Xianchun Xia.

[15]Mineral element concentrations in grains of Chinese wheat cultivars. Yong Zhang,Qichao Song,Jun Yan,Jianwei Tang,Rongrong Zhao,Yueqiang Zhang,Zhonghu He,Chunqin Zou,Ivan Ortiz-Monasterio. 2010

[16]Breeding Adult Plant Resistance to Stripe Rust in Spring Bread Wheat Germplasm Adapted to Sichuan Province of China. Zou, Y. C.,Yang, W. Y.,Tang, Y. L.,He, Z. H.,Singh, R. P.. 2011

[17]Cloning of 9-cis-epoxycarotenoid dioxygenase gene (TaNCED1) from wheat and its heterologous expression in tobacco. Zhang, S. J.,Song, G. Q.,Li, Y. L.,Gao, J.,Liu, J. J.,Fan, Q. Q.,Huang, C. Y.,Sui, X. X.,Chu, X. S.,Guo, D.,Li, G. Y.. 2014

[18]Development of dominant nuclear male-sterile lines with a blue seed marker in durum and common wheat. Liu, ZQ. 2001

[19]Inheritance in hexaploid wheat of genes for hairy auricles and hairy leaf sheath derived from Aegilops tauschii Coss.. Wu, BH,Hu, XR,Ye, Y,Zhang, Y. 1999

[20]A push-pull strategy to control aphids combines intercropping with semiochemical releases. Hatt, Severin,Zhang, Yong,Chen, Julian,Xu, Qinxuan,Hatt, Severin,Lopes, Thomas,Zhang, Yong,Francis, Frederic,Hatt, Severin,Bodson, Bernard. 2018

作者其他论文 更多>>

微信小程序