Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line

文献类型： 外文期刊

第一作者： Wu, Xiangju

作者： Wu, Xiangju;Wang, Jinbao;Zhao, Xiaomin;Wu, Xiangju;Qi, Jing;Cong, Xiaoyan;Chen, Lei;Hu, Yue;Sun, Wenbo;Chen, Zhi;Guo, Lihui;Wu, Jiaqiang;Li, Jun;Wang, Jinbao;Du, Yijun;Yoo, Dongwan;Wang, Guisheng;Li, Feng;Li, Feng

作者机构：

期刊名称：BIOMED RESEARCH INTERNATIONAL （ 影响因子：3.411； 五年影响因子：3.62 ）

ISSN： 2314-6133

年卷期： 2018 年

页码：

收录情况： SCI

摘要： Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV isolation and vaccine production. But MARC-145 cells have some limitations such as low virus yield. CD163 is a cellular receptor that mediates productive infection of PRRSV in various nonpermissive cell lines. In this study, we established a high and stable porcine CD163-(pCD163-) expressing MARC-145 cell line toward increasing its susceptibility to PRRSV infection. Indirect immunofluorescence assay (IFA) and Western blotting assays showed that pCD163 was expressed higher in pCD163-MARC cell line than MARC-145 cells. Furthermore, the ability of pCD163-MARC cell line to propagate PRRSV was significantly increased as compared with MARC-145 cells. Finally, we found that pCD163-MARC cell line had a higher isolation rate of clinical PRRSV samples and propagated live attenuated PRRS vaccine strains more efficiently than MARC-145 cells. This pCD163-MARC cell line will be a valuable tool for propagation and research of PRRSV.

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