Theoretical insights into the mechanism underlying aflatoxin B1 transformation by the BsCotA-methyl syringate system

文献类型： 外文期刊

第一作者： Wang, Xiaolu

作者： Wang, Xiaolu;Cui, Lin;Luo, Huiying;Huang, Huoqing;Tu, Tao;Qin, Xing;Wang, Yuan;Zhang, Jie;Wang, Yaru;Yao, Bin;Bai, Yingguo;Su, Xiaoyun;Liu, Mengting;Qi, Zheng

作者机构：

关键词： Laccase-Mediator System; Aflatoxin B 1; Methyl Syringate; Free Radical; Coupling

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.8； 五年影响因子：6.9 ）

ISSN： 0147-6513

年卷期： 2024 年 272 卷

页码：

收录情况： SCI

摘要： Global concern exists regarding the contamination of food and animal feed with aflatoxin B1 (AFB1), which poses a threat to the health of both humans and animals. Previously, we found that a laccase from Bacillus subtilis (BsCotA) effectively detoxified AFB1 in a reaction mediated by methyl syringate (MS), although the underlying mechanism has not been determined. Therefore, our primary objective of this study was to explore the detoxification mechanism employed by BsCotA. First, the enzyme and mediator dependence of AFB1 transformation were studied using the BsCotA-MS system, which revealed the importance of MS radical formation during the oxidation process. Aflatoxin Q1 (AFQ1) resulting from the direct oxidation of AFB1 by BsCotA, was identified using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The results of UPLC-MS/MS and density functional theory calculations indicated that the products included AFQ1, AFB1-, and AFD1-MS-coupled products in the BsCotA-MS system. The toxicity evaluations revealed that the substances derived from the transformation of AFB1 through the BsCotA-MS mechanism exhibited markedly reduced toxicity compared to AFB1. Finally, we proposed a set of different AFB1-transformation pathways generated by the BsCotA-MS system based on the identified products. These findings greatly enhance the understanding of the AFB1-transformation mechanism of the laccase-mediator system.

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