Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

文献类型： 外文期刊

第一作者： Xu, Xiao

作者： Xu, Xiao;You, Yinwei;Zhang, Long;You, Yinwei

作者机构：

关键词： Neuroscience;Issue 125;odorant receptor;RNA in situ hybridization;neuron;sensillum;insect antennae

期刊名称：JOVE-JOURNAL OF VISUALIZED EXPERIMENTS （ 影响因子：1.355； 五年影响因子：1.696 ）

ISSN： 1940-087X

年卷期： 2017 年 125 期

页码：

收录情况： SCI

摘要： Insects have evolved sophisticated olfactory reception systems to sense exogenous chemical signals. These chemical signals are transduced by Olfactory Receptor Neurons (ORNs) housed in hair-like structures, called chemosensilla, of the antennae. On the ORNs' membranes, Odorant Receptors (ORs) are believed to be involved in odor coding. Thus, being able to identify genes localized to the ORNs is necessary to recognize OR genes, and provides a fundamental basis for further functional in situ studies. The RNA expression levels of specific ORs in insect antennae are very low, and preserving insect tissue for histology is challenging. Thus, it is difficult to localize an OR to a specific type of sensilla using RNA in situ hybridization. In this paper, a detailed and highly effective RNA in situ hybridization protocol particularly for lowly expressed OR genes of insects, is introduced. In addition, a specific OR gene was identified by conducting double-color fluorescent in situ hybridization experiments using a co-expressing receptor gene, Orco, as a marker.

分类号：

  • 相关文献

[1]Pertussis toxin modulation of sodium channels in the central neurons of cyhalothrin-resistant and cyhalothrin-susceptible cotton bollworm, Helicoverpa armigera. Kong, De-Ling,He, Bing-Jun,Liu, Yan-Qiang,Fan, Xian-Lin,Liu, An-Xi.

[2]EXPRESSION OF SNMP1 AND SNMP2 GENES IN ANTENNAL SENSILLA OF Spodoptera exigua (HUBNER). Liu, Chengcheng,Zhang, Jin,Dong, Shuanglin,Liu, Chengcheng,Zhang, Jin,Liu, Yang,Wang, Guirong.

[3]Differential expression of two novel odorant receptors in the locust (Locusta migratoria). Xu, Haozhi,Guo, Mei,Yang, Ying,You, Yinwei,Zhang, Long,You, Yinwei. 2013

[4]Molecular identification and sex distribution of two chemosensory receptor families in Athetis lepigone by antennal transcriptome analysis. Zhang, Ya-Nan,Zhu, Xiu-Yun,Wang, Yi,Wang, Lu,Deng, Dao-Gui,Li, Jin-Bu,Ma, Ji-Fang,Dong, Zhi-Ping,Sun, Liang,Li, Zhao-Qun. 2016

[5]Gene structure and expression characteristic of a novel odorant receptor gene cluster in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae). Shan, S.,Zheng, Y.,Peng, Y.,Yang, Y. -Q.,Zhang, Y. -J.,Guo, Y. -Y.,Shan, S.,Zheng, Y.,Yang, Y. -Q.,Li, R. -J.,Lu, Z. -Y..

[6]A broadly tuned odorant receptor in neurons of trichoid sensilla in locust, Locusta migratoria. You, Yinwei,Lv, Mingyue,Zhang, Long,You, Yinwei,Smith, Dean P.,Smith, Dean P..

[7]A Female-Biased Odorant Receptor from Apolygus lucorum (Meyer-Dur) Tuned to Some Plant Odors. Zhang, Meiping,Zhang, Zhixiang,Yan, Shuwei,Wang, Guirong,Liu, Yang. 2016

[8]Identification and Expression Analysis of Putative Chemosensory Receptor Genes in Microplitis mediator by Antennal Transcriptome Screening. Dhiloo, Khalid Hussain,Gu, Shao-Hua,Zhang, Yong-Jun,Guo, Yu-Yuan,Peng, Yong,Li, Rui-Jun,Lu, Zi-Yun,Zhou, Jing-Jiang,Dhiloo, Khalid Hussain. 2015

[9]An odorant receptor from the common cutworm (Spodoptera litura) exclusively tuned to the important plant volatile cis-3-Hexenyl acetate. Liu, C. C.,Yan, S. W.,Dong, S. L.,Zhang, J.,Liu, C. C.,Liu, Y.,Guo, M. B.,Wang, G. R.. 2013

[10]Knockdown of Microplitis mediator Odorant Receptor Involved in the Sensitive Detection of Two Chemicals. Ren, Li-Yan,Zhang, Yong-Jun,Wu, Kong-Ming,Guo, Yu-Yuan,Li, Ke-Ming. 2012

[11]Molecular characterization and differential expression of two duplicated odorant receptor genes, AcerOr1 and AcerOr3, in Apis cerana cerana. Zhao, Huiting,Gao, Pengfei,Du, Haiyan,Ma, Weihua,Tian, Songhao,Jiang, Yusuo,Ma, Weihua.

作者其他论文 更多>>

微信小程序