Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapa L. ssp pekinensis) and Molecular Marker Development

文献类型： 外文期刊

第一作者： Li, Jingjuan

作者： Li, Jingjuan;Zhang, Yihui;Ding, Qian;Li, Huayin;Liu, Lifeng;Wang, Fengde;Gao, Jianwei

作者机构：

期刊名称：INTERNATIONAL JOURNAL OF GENOMICS （ 影响因子：2.326； 五年影响因子：2.557 ）

ISSN： 2314-436X

年卷期： 2017 年

页码：

收录情况： SCI

摘要： Due to the visual appearance and high carotenoid content, orange inner leaves are a desirable trait for the Chinese cabbage. To understand the molecular mechanism underlying the formation of orange inner leaves, the BrCRTISO (Bra031539) gene, as the Br-or candidate gene, was analyzed among the white and orange varieties, and 7 single nucleotide polymorphisms (SNPs) were identified. However, only one SNP (C-952 to T-952) altered the amino acid sequence, resulting in a mutation from Leu(318) to Phe(318) in the orange varieties. Additionally, we analyzed differentially expressed genes (DEGs) between the orange and white F-2 individuals (14-401 x 14-490) and found four downregulated genes were involved in the carotenoid biosynthesis pathway, which may lead to the accumulation of prolycopene and other carotenoid pigments in the orange inner leaves. In addition, we developed a novel InDel marker in the first intron, which cosegregates with the phenotypes of orange color inner leaves. In conclusion, these findings enhance our understanding of the underlying mechanism of pigment accumulation in the inner leaves of the Chinese cabbage. Additionally, the SNP (C-952 to T-952) and the InDel marker will facilitate the marker-assisted selection during Chinese cabbage breeding.

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