Development and Preliminary Application of an Indirect ELISA to Detect Infectious Bovine Rhinotracheitis Virus Using Recombinant Glycoprotein D of IBRV Strain SD

文献类型： 外文期刊

第一作者： Song, Lingling

作者： Song, Lingling;Zhang, Hui;Hou, Peili;Wang, Hongmei;Zhao, Guimin;He, Hongbin;Xia, Xianzhu

作者机构：

关键词： gD;Indirect ELISA;Infectious bovine rhinotracheitis virus;Serology

期刊名称：KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI （ 影响因子：0.685； 五年影响因子：0.655 ）

ISSN： 1300-6045

年卷期： 2016 年 22 卷 4 期

页码：

收录情况： SCI

摘要： Glycoprotein D (gD) is the major structural protein of infectious bovine rhinotracheitis virus (IBRV). It can induce both humoral and cellular immunity thus it is a preferable protein for IBR diagnostic reagent. Regarding to DNAstar analysis, the major antigenic region of the gD fragment was amplified by PCR using the IBRV genomic DNA as a template, and subsequenly constructed into the recombinant plasmid pET32a-gD. The fusion protein was expressed upon IPTG induction. The fusion protein was purified by immobilized Ni ion affinity chromatography with a Ni-NTA Kit after verification by SDS-PAGE and Western-blotting analysis, then was utilized as a coating antigen to detect antibodies to infectious bovine rhinotracheitis virus (IBRV) in an indirect ELISA method. Cross-reactivity examinations have showed that the recombinant antigen had no cross reaction with positive sera of other common viral diseases (bovine ephemeral fever, bovine viral diarrhea-mucosal disease, calf diarrhea, bovine intestinal virus infection, bovine coronavirus disease) which indicating a strong specificity. Application of this diagnostic method in 1315 clinical serum samples displayed an antibody positive rate of 23.7% (311/1315), with respect to a coincidence rate of 96.8% as compared to a commercialized IBRV whole virus ELISA. Our method is stable and sensitive hence provides a quick and convenient serological diagnosis favoring epidemiology and disease identification of domestic IBR.

分类号：

  • 相关文献

[1]RNA interference targeting glycoprotein D inhibits infectious bovine rhinotracheitis virus replication in MDBK cells. Song, Lingling,Zhang, Hui,Wang, Hongmei,Hou, Peili,Xia, Xianzhu,He, Hongbin. 2016

[2]A monoclonal antibody-blocking enzyme-linked immunosorbent assay for the detection of serovar-specific antibodies to Haemophilus paragallinarum. Zhang, P,Blackall, PJ,Yamaguchi, T,Iritani, Y. 1999

[3]Identification and characterization of a new cucumber mosaic virus isolate from passionfruit in Hainan of China. Liu, ZX,Pan, JS,Wei, YW,Wu, H,Zheng, XQ. 2004

[4]The double-antigen ELISA concept for early detection of E-rns-specific classical swine fever virus antibodies and application as an accompanying test for differentiation of infected from marker vaccinated animals. Meyer, D.,Becher, P.,Postel, A.,Fritsche, S.,Engemann, C.,Luo, Y.,Qiu, H. -J.,Blome, S.,Beyerbach, M.,Chang, C. -Y.. 2017

[5]First report of Rickettsia felis in China. Zhang, Jilei,Lu, Guangwu,Wei, Lanjing,Wang, Chengming,Kelly, Patrick,Zhang, Zhenwen,Yu, Duonan,Kayizha, Shayilan. 2014

[6]Biological and Molecular Characterization of a Crucifer Tobamovirus Infecting Oilseed Rape. Cai, Li,Hou, Mingsheng,Cai, Li,Chen, Kunrong,Zhang, Xuejiang,Yan, Liying,Xu, Zeyong. 2009

[7]Use of serological and mucosal immune responses to Mycoplasma hyopneumoniae antigens P97R1, P46 and P36 in the diagnosis of infection. Feng, Zhi-Xin,Bai, Yun,Yao, Jing-Ting,Gan, Yuan,Wang, Hai-Yan,Wei, Yan-Na,Liu, Mao-Jun,Xiong, Qi-Yan,Bai, Fang-Fang,Li, Bin,Wu, Xu-Su,Shao, Guo-Qing,Feng, Zhi-Xin,Bai, Yun,Liu, Mao-Jun,Shao, Guo-Qing,Pharr, G. Todd,Wan, Xiu-Feng,Xiao, Shao-Bo,Chi, Ling-Zhi.

[8]Characterization and partial sequence of a new furovirus of wheat in China. Ye, R,Zheng, T,Chen, J,Diao, A,Adams, MJ,Yu, S,Antoniw, JF.

[9]Performance of Indirect ELISA for Maize Hybrid Purity Test. Liu, Yuehui,Wang, Jun,Yang, XinChao,Sun, Naxin,Xu, Lihua. 2013

[10]A novel fusion protein-based indirect enzyme-linked immunosorbent assay for the detection of bovine tuberculosis. Liu, Siguo,Guo, Sheping,Wang, Chunlai,Shao, Meili,Zhang, Xiuhua,Guo, Yang,Gong, Qiang. 2007

[11]Prokaryote expression of HPeV-1 VP1 protein, production of VP1 polyclonal antibody and the development of an ELISA. Yu, Ying,Shan, Tongling,Zhu, Caixia,Hua, Xiuguo,Shan, Tongling,Wang, Chunmei,Zhu, Shaofeng,Wang, Chunmei.

[12]An indirect ELISA for detection of Theileria spp. antibodies using a recombinant protein (rTlSP) from Theileria luwenshuni. He, Haining,Li, Youquan,Liu, Junlong,Liu, Zhijie,Yang, Jifei,Liu, Aihong,Chen, Ze,Ren, Qiaoyun,Guan, Guiquan,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong.

[13]Development of an indirect ELISA for serological detection of reticuloendotheliosis virus using the gp90 protein expressed in Pichia pastoris. Li, Kai,Gao, Honglei,Gao, Li,Qi, Xiaole,Gao, Yulong,Qin, Liting,Wang, Yongqiang,Wang, Xiaomei.

[14]Detection of the Soybean Allergenic Protein Gly m Bd 28K by an Indirect Enzyme-Linked Immunosorbent Assay. Liu, Bin,Teng, Da,Wang, Xiumin,Wang, Jianhua,Liu, Bin,Teng, Da,Wang, Xiumin,Wang, Jianhua.

[15]Development of an Indirect ELISA with Artificially Synthesized N Protein of PPR Virus. Zhu, Yu-min,Dong, Shi-juan,Yu, Rui-song,Li, Zhen,Zhu, Yu-min,Dong, Shi-juan,Yu, Rui-song,Li, Zhen,Zhang, Guo-rui,Lei, Zhi-hai,Zeng, Jiang-yong,Zhu, Se,Duoji, Ciren. 2012

[16]Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection. Ge, Junwei,Cui, Xingyang,Shi, Yunjia,Wei, Chengwei,Wen, Shanshan,Xia, Shuang,Zhao, Lili,Chen, Hongyan,Ge, Junwei. 2018

[17]Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA. Liu, Rongchang,Chen, Cuiteng,Cheng, Longfei,Lu, Ronghui,Fu, Guanghua,Shi, Shaohua,Chen, Hongmei,Wan, Chunhe,Lin, Jiansheng,Fu, Qiuling,Huang, Yu. 2017

[18]Expression of the soybean allergenic protein P34 in Escherichia coli and its indirect ELISA detection method. Liu, Bin,Teng, Da,Wang, Xiumin,Yang, Yalin,Wang, Jianhua,Liu, Bin,Teng, Da,Wang, Xiumin,Yang, Yalin,Wang, Jianhua. 2012

[19]Differential Diagnosis of Antibody to Classical Swine Fever Virus Field Strain by ELISA with Recombinant E2 Proteins of Various Group CSFV. Qiu Chang-Qing,Hu Hui,Cao Xiao-An,Zhou Ji-Zhang,Lin Guo-Zhen,Hu Hui. 2008

[20]Development and application of G(1)-ELISA for detection of antibodies against bovine ephemeral fever virus. Zheng, Fu-ying,Lin, Guo-zhen,Qiu, Chang-qing,Zhou, Ji-zhang,Cao, Xiao-an,Gong, Xiao-wei. 2009

作者其他论文 更多>>

微信小程序