Fosmid library construction and screening for the maize mutant gene Vestigial glume 1

文献类型： 外文期刊

第一作者： Liu, Chaoxian

作者： Liu, Chaoxian;Liu, Xiaoli;Cai, Yilin;Lei, Lei;Guan, Haiying

作者机构：

关键词： Vestigial glume 1;Fosmid;Library construction;Library screening

期刊名称：CROP JOURNAL （ 影响因子：4.407； 五年影响因子：5.687 ）

ISSN： 2095-5421

年卷期： 2016 年 4 卷 1 期

页码：

收录情况： SCI

摘要： The maize mutant gene Vestigial glume 1 (Vg1) has been fine-mapped to a narrow region by map-based cloning and the candidate gene for Vg1 spanned 19.5 kb. Here we report Vg1 genomic fosmid library construction and screening. The fosmid library of Vg1 consisted of 574,000 clones with an average insert size of 36.4 kb, representing 7.9-fold coverage of the maize genome. Fosmid stability assays indicated that clones were stable during propagation in the fosmid system. Using Vg1 candidate gene-specific primers, a positive clone was successfully identified. This discovery will pave the way for identifying the function of Vg1 in maize development. (C) 2015 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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