The blocking reagent optimization for the magnetoelastic biosensor

文献类型： 外文期刊

第一作者： Hu, Jiajia

作者： Hu, Jiajia;Hu, Jing;Hu, Jiajia;Chai, Yating;Horikawa, Shin;Wikle, Howard C.;Du, Songtao;Chin, Bryan A.;Wang, Feng'en

作者机构：

关键词： magnetoelastic biosensor;blocking reagents;Salmonella. Typhimurium

期刊名称：SENSING FOR AGRICULTURE AND FOOD QUALITY AND SAFETY VII

ISSN： 0277-786X

年卷期： 2015 年 9488 卷

页码：

收录情况： SCI

摘要： The wireless phage-based magnetoelastic (ME) biosensor has proven to be promising for real-time detection of pathogenic bacteria on fresh produces. The ME biosensor consists of a freestanding ME resonator as the signal transducer and filamentous phage as the biomolecular-recognition element, which can specifically bind to a pathogen of interest. Due to the Joule magnetostriction effect, the biosensors can be placed into mechanical resonance when subjected to a time-varying magnetic field alternating at the sensor's resonant frequency. Upon the attachment of the target pathogen, the mass of the biosensor increases, thereby decreasing its resonant frequency. This paper presents an investigation of blocking reagents immobilization for detecting Salmonella Typhimurium on fresh food surfaces. Three different blocking reagents (BSA, SuperBlock blocking buffer, and blocker BLOTTO) were used and compared. The optical microscope was used for bacterial cells binding observation. Student t-test was used to statistically analysis the experiment results. The results shows that SuperBlock blocking buffer and blocker BLOTTO have much better blocking performance than usually used BSA.

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