Physiological responses to heat stress in the liver of rainbow trout (Oncorhynchus mykiss) revealed by UPLC-QTOF-MS metabolomics and biochemical assays
文献类型： 外文期刊
第一作者： Li, Shanwei
作者： Li, Shanwei;Liu, Yingjie;Ding, Lu;Wei, Xiaofeng;Wang, Peng;Chen, Zhongxiang;Han, Shicheng;Huang, Tianqing;Wang, Bingqian;Sun, Yanchun;Li, Shanwei;Liu, Yingjie;Ding, Lu;Sun, Yanchun;Li, Bolun;Wei, Xiaofeng
作者机构：
关键词： Heat stress; Rainbow trout; Metabolomics; Antioxidant defense; Immune response
期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：7.129； 五年影响因子：7.284 ）
ISSN： 0147-6513
年卷期： 2022 年 242 卷
页码：
收录情况： SCI
摘要： Rainbow trout (Oncorhynchus mykiss) is one of the world's most widely farmed cold-water fish. However, the rise in water temperature caused by global warming has seriously restricted the development of rainbow trout aquaculture. In this study, we investigated the physiological responses in the liver of rainbow trout exposed to 20 degrees C and 24 degrees C and returning to the initial temperature (14 degrees C) by combining biochemical analyses and UPLC-QTOF-MS metabolomics. The results of the biochemical analysis showed that serum aminotransferase, lysozyme, total bilirubin, alkaline phosphatase and liver superoxide dismutase, glutathione peroxidase, and malondialde-hyde in rainbow trout under heat stress changed significantly. Even after the temperature recovery, some of the above indicators were still affected. Compared to the control group, 115, 130, and 121 differentially expressed metabolites were identified in the 20 degrees C, 24 degrees C, and recovery groups, respectively. Further pathway enrichment of these metabolites revealed that heat stress mainly affected the linoleic acid metabolism, alpha-linolenic acid metabolism, glycerophospholipid metabolism, and sphingolipid metabolism in the liver of rainbow trout, and continuously affected these metabolic pathways during the recovery period. Notably, the enrichment of gluta-thione metabolic pathways was consistent with the changes in glutathione peroxidase in the biochemical results. The results above suggest that heat stress can induce immune responses and oxidative stress inside the rainbow trout. After temperature recovery, some of the hepatic functions of fish return to normal gradually. The biochemical analysis and UPLC-QTOF-MS metabolomics tools provide insight into the physiological regulation of rainbow trout in response to heat stress.
分类号：
- 相关文献
作者其他论文 更多>>
-
Telomere-to-telomere genome assembly of eggplant ( Solanum melongena L.) promotes gene fine localization of the green stripe (GS) in pericarp
作者：Fang, Huarong;Wu, Jie;Xie, Liang;Li, Yizhiqi;Huang, Jingjing;Yan, Xiaoyun;He, Xuehan;Deng, Wentao;Li, Ruirui;Yu, Wenjin;Wang, Peng;Chen, Jie;Ji, Yanhai;Wen, Changlong
关键词：Eggplant; T2T genome; Green stripe
-
Transcriptomic Insights into Dual Temperature-Salinity Stress Response in "Shuike No. 1", a Pioneering Rainbow Trout Strain Bred in China
作者：Liu, Xiaojun;Wang, Gaochao;Huang, Tianqing;Liu, Enhui;Gu, Wei;Fan, Peng;Ge, Kaibo;Li, Datian;Sun, Yunchao;Xu, Gefeng;Liu, Xiaojun;Wang, Gaochao;Huang, Tianqing;Liu, Enhui;Gu, Wei;Fan, Peng;Ge, Kaibo;Li, Datian;Sun, Yunchao;Xu, Gefeng;Liu, Xiaojun;Wang, Gaochao;Huang, Tianqing;Liu, Enhui;Gu, Wei;Fan, Peng;Ge, Kaibo;Li, Datian;Sun, Yunchao;Xu, Gefeng;Liu, Xiaojun
关键词：rainbow trout; salinity stress; temperature stress; RNA-seq; WGCNA
-
Evaluation of Two Different Treatments for Larch Logs as Substrates to Cultivate Ganoderma tsugae in the Forest
作者：Xia, Lei;Tan, Xiao;Wang, Peng;Yang, Dahai;Zhang, Yang;Cui, Yanru;Yu, Ya;Zhang, Weidong;Huang, Xiao;Wen, Jiawei
关键词：
Ganoderma tsugae ; larch logs; yield; agronomic traits; nutritional value -
Genome-Enabled Analyses Reveal Roles of Mangrove Homeobox Superfamily in Response to Salinity
作者：Chen, Tingting;Chen, Tingting;Ye, Xiuxu;Wang, Peng;Feng, Erhui;Zhang, Yu;Wang, Peng
关键词：Homeobox superfamily; Rhizophoraceae; Mangrove; Salinity stress
-
Multi-generational adaptation to Solanum nigrum increases reproduction and decreases microbial diversity of Aphis gossypii
作者：Wang, Peng;Ma, Ya-Jie;Wang, Dan;Shan, Yong-Pan;Hu, Hongyan;Wu, Changcai;Song, Xian-Peng;Ren, Xiangliang;Ma, Yan;Ma, Xiaoyan;Wang, Peng;Jing, Yu-Xi;Ren, Xiangliang;Ma, Yan;Ma, Xiaoyan;Ma, Ya-Jie;Ren, Xiangliang;Ma, Yan;Ma, Xiaoyan
关键词：cotton aphid; 16S rRNA; life table; host shift; symbiotic bacterium
-
Exploring Disparities in Gill Physiological Responses to NaHCO3-Induced Habitat Stress in Triploid and Diploid Crucian Carp (Carassius auratus): A Comprehensive Investigation Through Multi-Omics and Biochemical Analyses
作者：Han, Shicheng;Han, Lin;Yuan, Fangying;Liu, Wenzhi;Jin, Xiaofeng;Sun, Yanchun;Han, Lin;Liu, Wenzhi;Yuan, Fangying;Wang, Jing;Jin, Xiaofeng
关键词：crucian carp (
Carassius auratus ); diploid; triploid; carbonate alkalinity; metabolomics; transcriptomics -
Whole-genome resequencing reveals selection signatures in China's first rainbow trout (Oncorhynchus mykiss) strain "Shuike No. 1"
作者：Ma, Haibing;Wang, Gaochao;Huang, Tianqing;Liu, Enhui;Gu, Wei;Xu, Gefeng;Ma, Haibing;Xu, Gefeng
关键词：Rainbow trout; Population structure; Selective sweep; Linkage disequilibrium
