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Cloning of 9-cis-epoxycarotenoid dioxygenase gene (TaNCED1) from wheat and its heterologous expression in tobacco

文献类型：外文期刊

第一作者： Zhang, S. J.

作者： Zhang, S. J.;Song, G. Q.;Li, Y. L.;Gao, J.;Liu, J. J.;Fan, Q. Q.;Huang, C. Y.;Sui, X. X.;Chu, X. S.;Guo, D.;Li, G. Y.

作者机构：

关键词： abscisic acid;drought tolerance;low temperature;Nicotiana tabacum;Triticum aestivum

期刊名称：BIOLOGIA PLANTARUM （影响因子：1.747；五年影响因子：2.146 ）

ISSN： 0006-3134

年卷期： 2014 年 58 卷 1 期

页码：

收录情况： SCI

摘要： Abscisic acid (ABA) regulates plant responses to various environmental stresses. Oxidative cleavage of cis-epoxycarotenoids catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED) is the critical step in the biosynthesis of ABA in higher plants. Using a homologous cloning approach, a NCED-like gene (designated as TaNCED1) was isolated from wheat (Triticum aestivum). It contained an open reading frame of 1 848 bp and encodes a peptide of 615 amino acids. Multiple sequence alignments showed that TaNCED1 shared high identity with NCEDs from other plants. Phylogenetic analysis revealed that TaNCED1 was most closely related to a barley HvNCED1 gene. The predicted 3D structure of TaNCED1 showed high similarity with other homologues. Southern blot analysis indicated that TaNCED1 was a single copy in the genome of wheat. TaNCED1 was differentially expressed in various organs and the expression was up-regulated by low temperature, drought, NaCl, and ABA. Heterologous expression of TaNCED1 in tobacco (Nicotiana tabacum) significantly improved its drought tolerance. Under drought treatment, TaNCED1-overexpressing transgenic tobacco plants exhibited higher germination rate, higher relative water content, content of soluble sugars and of ABA when compared with the wild type plants.

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