Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp PCC6803

文献类型： 外文期刊

第一作者： Chen, Gao

作者： Chen, Gao;Bian, Fei;Peng, Zhenying;Zhang, Yan;Yu, Jinhui;Xuan, Ning;Bi, Yuping;He, Qingfang;Chen, Gao;Bian, Fei;Peng, Zhenying;Zhang, Yan;Yu, Jinhui;Xuan, Ning;Bi, Yuping;He, Qingfang;He, Qingfang;Wang, Qiang;Qu, Shujie;Ge, Haitao

作者机构：

关键词： Omega-3 polyunsaturated fatty acids;Gene expression;Fatty acid desaturase;Synechocystis sp PCC6803

期刊名称：BIOTECHNOLOGY FOR BIOFUELS （ 影响因子：6.04； 五年影响因子：6.485 ）

ISSN： 1754-6834

年卷期： 2014 年 7 卷

页码：

收录情况： SCI

摘要： Background: Polyunsaturated fatty acids (PUFAs), which contain two or more double bonds in their backbone, are the focus of intensive global research, because of their nutritional value, medicinal applications, and potential use as biofuel. However, the ability to produce these economically important compounds is limited, because it is both expensive and technically challenging to separate omega-3 polyunsaturated fatty acids (omega-3 PUFAs) from natural oils. Although the biosynthetic pathways of some plant and microalgal omega-3 PUFAs have been deciphered, current understanding of the correlation between fatty acid desaturase content and fatty acid synthesis in Synechocystis sp. PCC6803 is incomplete. Results: We constructed a series of homologous vectors for the endogenous and exogenous expression of Delta 6 and Delta 15 fatty acid desaturases under the control of the photosynthesis psbA2 promoter in transgenic Synechocystis sp. PCC6803. We generated six homologous recombinants, harboring various fatty acid desaturase genes from Synechocystis sp. PCC6803, Gibberella fujikuroi and Mortierella alpina. These lines produced up to 8.9 mg/l of alpha-linolenic acid (ALA) and 4.1 mg/l of stearidonic acid (SDA), which are more than six times the corresponding wild-type levels, at 20 degrees C and 30 degrees C. Thus, transgenic expression of Delta 6 and Delta 15 fatty acid desaturases enhances the accumulation of specific omega-3 PUFAs in Synechocystis sp. PCC6803. Conclusions: In the blue-green alga Synechocystis sp. PCC6803, overexpression of endogenous and exogenous genes encoding PUFA desaturases markedly increased accumulation of ALA and SDA and decreased accumulation of linoleic acid and gamma-linolenic acid. This study lays the foundation for increasing the fatty acid content of cyanobacteria and, ultimately, for producing nutritional and medicinal products with high levels of essential omega-3 PUFAs.

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