Associating bovine herpesvirus 1 envelope glycoprotein gD with activated phospho-PLC-λ1(S1248)

文献类型： 外文期刊

第一作者： Liu, Chang

作者： Liu, Chang;Yang, Hao;Tang, Linke;Zhao, Heci;Ding, Xiuyan;Zhu, Liqian;Yuan, Weifeng;Ni, Junqing;Neumann, Donna;Zhu, Liqian

作者机构：

关键词： bovine herpesvirus 1; phospholipase C-& gamma;1; Golgi apparatus; gD

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.7； 五年影响因子：5.9 ）

ISSN： 2165-0497

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Phospholipase C gamma 1 (PLC-gamma 1) may locate at distinct subcellular locations, such as cytosol, plasma membrane, and nucleus for varied biological functions. Bovine herpesvirus 1 (BoHV-1) productive infection activates PLC-gamma 1 signaling, as demonstrated by increased protein levels of phosphorylated-PLC-.1 at Ser1248 [p-PLC-.1(S1248)], which benefits virus productive infection. Here, for the first time, we reported that Golgi apparatus also contains activated p-PLC-gamma 1(S1248). And BoHV-1 productive infection at later stages (24 hpi) increased the accumulation of p-PLC.1(S1248) in the Golgi apparatus, where p-PLC-gamma 1(S1248) forms highlighted puncta observed via a confocal microscope. Coimmunoprecipitation studies demonstrated that the Golgi p-PLC-gamma 1(S1248) is specifically associated with the viral protein gD but not gC. In addition, we found that p-PLC-gamma 1(S1248) is consistently associated with both the plasma membrane-associated virions and the released virions. When the virus-infected cells were treated with PLC-gamma 1-specific inhibitor, U73122, for a short duration of 4 hours prior to the endpoint of virus infection, we found that the viral protein gD was trapped in the Golgi apparatus, suggesting that the PLC-gamma 1 signaling may facilitate traffickingtraffickingtraffickingof progeny virions out of this organelle. These findings provide a novel insight into the interplay between PLC-gamma 1 signaling and BoHV-1 replication.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序