The Caspase Pathway of Linoelaidic Acid (9t, 12t-C18:2)-Induced Apoptosis in Human Umbilical Vein Endothelial Cells

文献类型： 外文期刊

第一作者： Bin, Qiu

作者： Bin, Qiu;Rao, Huan;Hu, Jiang-Ning;Liu, Rong;Fan, Ya-Wei;Li, Jing;Deng, Ze-Yuan;Zhong, Xianfeng;Bin, Qiu;Du, Fang-Ling

作者机构：

关键词： Linoelaidic acid;Human umbilical vein endothelial cells;Caspase;Apoptosis

期刊名称：LIPIDS （ 影响因子：1.88； 五年影响因子：2.417 ）

ISSN： 0024-4201

年卷期： 2013 年 48 卷 2 期

页码：

收录情况： SCI

摘要： Trans fatty acids (TFA) are reported to contribute to inflammation and coronary heart disease. The study aim was to investigate the proapoptotic effects of two double bond TFA (TDTFA) on human umbilical vein endothelial cells (HUVEC). The HUVEC were grown in media supplied with linoelaidic acid (9t,12t-C18:2) at 50, 100, 200, 400 mu mol/l for 24 or 48 h to examine the effects of TDTFA on the viability and apoptosis of these cells. Flow cytometry analysis and confocal scanning were used to measure apoptosis, cell binding of Annexin V and propidium iodide uptake. Colorimetric assay and RT-PCR were used to analyze enzyme activities and mRNA expression of caspase-3, -8 and -9 in HUVEC. Results showed that 9t,12t-C18:2 inhibited the viability of HUVEC in a dose-dependent and time-dependent manner. The percentages of 9t,12t-C18:2 induced apoptotic and necrotic cells significantly increased compared with that of the control. The activities and mRNA expression of caspase-8, -9 and -3 were significantly increased in 9t,12t-C18:2 treated cells compared to that of the control. Addition of specific inhibitors of caspase-8 (z-IETD-fmk) and caspase-9 (z-LEHD-fmk) to HUVEC was found to completely inhibit 9t,12t-C18:2-induced activation of caspase-3, and z-IETD-fmk inhibited the activation of caspase-9. Meanwhile, it was found that mRNA expression of Bid, Smac/DIABLO and the release of mitochondrial cytochrome c were significantly elevated by 9t,12t-C18:2 treatment. These results suggest that 9t,12t-C18:2 may induce apoptosis of HUVEC through activating caspase-8, -9 and -3. Both the death receptor pathway and the mitochondrial pathway may be involved in the apoptosis induced by 9t,12t-C18:2.

分类号：

  • 相关文献

[1]Characterization of three caspases and their pathogen-induced expression pattern in Portunus trituberculatus. Ren, Xianyun,Yu, Xuan,Gao, Baoquan,Liu, Ping,Li, Jian,Ren, Xianyun,Yu, Xuan,Gao, Baoquan,Liu, Ping,Li, Jian,Yu, Xuan.

[2]Strophalloside Induces Apoptosis of SGC-7901 Cells through the Mitochondrion-Dependent Caspase-3 Pathway. Zhang, Xue-Jiao,Mei, Wen-Li,Dai, Hao-Fu,Zhang, Xue-Jiao,Tan, Guang-Hong,Wang, Cai-Chun,Zhou, Song-Lin,Huang, Feng-Ru,Chen, Bin,Huang, Feng-Ying,Zhang, Xue-Jiao.

[3]Effects of camptothecin and hydroxycamptothecin on insect cell lines Sf21 and IOZCAS-Spex-II. Zhang, Lan,Zhang, Yanning,He, Weizhi,Ma, Dejun,Jiang, Hongyun. 2012

[4]AlCl3 induces lymphocyte apoptosis in rats through the mitochondria-caspase dependent pathway. Li, Miao,Song, Miao,Ren, Li-Min,Xiu, Chun-Yu,Liu, Jian-Yu,Li, Yan-Fei,Liu, Jian-Yu,Zhu, Yan-zhu.

[5]The comparative study among different fractions of muscadine grape 'Noble' pomace extracts regarding anti-oxidative activities, cell cycle arrest and apoptosis in breast cancer. Song, Shiren,Lu, Jiang,Luo, Jianming,Wei, Zheng,Zhang, Yali,Luo, Jianming,Wei, Zheng,Huang, Yu,Lu, Jiang. 2017

[6]Effects of Dietary Protein and Calcium on Thymus Apoptosis Induced by Fluoride in Female Rats (Wistar Rats). Wang, Hongwei,Zhou, Bianhua,Cao, Jinling,Cao, Chunfang,Wang, Jundong,Zhou, Bianhua,Gu, Xinli.

[7]Antiangiogenic activity of low-temperature lysozyme from a marine bacterium in vivo and in vitro. Wang Zhenhua,Liu Jincheng,Su Ai,Wang Chunbo,Sun Mi. 2009

[8]The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endothelium: a review. Cao, Yi,Gong, Yu,Zhou, Yiwei,Fang, Xin,Zhang, Cao,Li, Yining,Li, Juan,Cao, Yi,Liu, Liangliang,Zhou, Yiwei,Fang, Xin. 2017

[9]Influence of oxidation on the susceptibility of purified desmin to degradation by mu-calpain, caspase-3 and-6. Chen, Qianqian,Huang, Jichao,Huang, Ming,Zhou, Guanghong,Huang, Feng. 2014

[10]Cleavage of the calpain inhibitor, calpastatin, during postmortem ageing of beef skeletal muscle. Huang, Feng,Zhang, Hong,Zhang, Dequan,Huang, Ming,Guo, Bing,Zhou, Guanghong.

[11]Detection of DNA ladder during cotyledon senescence in cotton. Q.E. XIE,I.D. LIU,S.X. YU,R.F. WANG,Z.X. FAN,Y.G. WANG,F.F. SHEN.

[12]Brusatol isolated from Brucea javanica (L.) Merr. induces apoptotic death of insect cell lines. Feng, Xuehuan,Ma, Dejun,Yang, Jingjing,Jiang, Hongyun,Zhang, Yanning,He, Weizhi. 2013

[13]Antiproliferative Effect of Newcastle Disease Virus Strain D90 on Human Lung Cancer Cell Line A549. Fu, Fang,Li, Xi,Zhao, Ming,Yang, Bao-Feng,Song, Chun,Yang, Yu-Ju,Tong, Guang-Zhi. 2011

[14]Cytotoxicity of melittin and apamin in human hepatic L02 and HepG2 cells in vitro. Zhou, Jinhui,Diao, Qingyun,Wu, Liming,Du, Xia,Li, Yi,Sun, Liping,Zhou, Jinhui,Wu, Liming,Li, Yi,Zhou, Jinhui,Wu, Liming,Li, Yi,Qi, Yitao.

[15]Chlorpyrifos is estrogenic and alters embryonic hatching, cell proliferation and apoptosis in zebrafish. Yu, Kaimin,Li, Guochao,Feng, Weimin,Liu, Lili,Zhang, Jiayu,Wu, Wei,Xu, Lei,Yan, Yanchun.

[16]In vitro protective efficacy of Lithium chloride against Mycoplasma hyopneumoniae infection. Ishag, Hassan Z. A.,Wu, Yu-zi,Liu, Mao-jun,Xiong, Qi-yan,Feng, Zhi-xin,Yang, Ruo-song,Shao, Guo-qing,Ishag, Hassan Z. A..

[17]Dietary vitamin A supplementation improved reproductive performance by regulating ovarian expression of hormone receptors, caspase-3 and Fas in broiler breeders. Chen, Fang,Chen, Fang,Jiang, Zongyong,Jiang, Shouqun,Li, Long,Lin, Xiajing,Gou, Zongyong,Fan, Qiuli.

[18]Apoptotic effects of hydrogen peroxide and vitamin C on chicken embryonic fibroblasts: redox state and programmed cell death. Jin, D. P.,Zhang, W. X.,Li, C. L.,Guan, W. J.,Ma, Y. H.,Jin, D. P.,Yang, H. J.,Li, C. Y..

[19]Thapsigargin induces apoptosis when autophagy is inhibited in HepG2 cells and both processes are regulated by ROS-dependent pathway. Wang, Congcong,Tang, Shusheng,Zhao, Dongxu,Zhang, Chaoming,Zhang, Shen,Deng, Sijun,Zhou, Yan,Xiao, Xilong,Wang, Congcong,Li, Tao.

[20]The core structure of a Dendrobium huoshanense polysaccharide required for the inhibition of human lens epithelial cell apoptosis. Zha, Xue-Qiang,Zha, Xue-Qiang,Li, Xiao-Long,Wang, Jing-Fei,Pan, Li-Hua,Luo, Jian-Ping,Deng, Yuan-Yuan.

作者其他论文 更多>>

微信小程序