Development of Ty1-copia retrotransposon-based S-SAP markers in strawberry (Fragaria x ananassa Duch.)

文献类型: 外文期刊

第一作者: He, Ping

作者: He, Ping;Ma, Yue;Dai, Hongyan;Liu, Yuexue;Li, He;Zhang, Zhihong;He, Ping;Li, Linguang;Zhao, Guiling

作者机构:

关键词: FaRE1 retrotransposon;Strawberry;S-SAP;Polymorphism

期刊名称:SCIENTIA HORTICULTURAE ( 影响因子:3.463; 五年影响因子:3.672 )

ISSN: 0304-4238

年卷期: 2012 年 137 卷

页码:

收录情况: SCI

摘要: The most popular retrotransposon-based molecular marker system at the present time is the sequence-specific amplification polymorphism (S-SAP) system, which is widely used for various applications including phylogenetic analysis. In this study, we successfully developed a new S-SAP marker system based on FaRE1 retrotransposon in strawberry genome. Among 34 primer combinations used to develop the S-SAP marker system, seven primer combinations produced high quality and reliability banding patterns. A total of 288 polymorphic bands from 722 amplified bands were detected in 13 strawberry accessions, with a polymorphic ratio of 39.8%. Phylogenetic analysis showed that genetic coefficient ranged from 0.67 to 1.00 for 13 strawberry accessions. An 187 bp S-SAP band was detected from 50 strawberry plantlets regenerated from leaves which were cultured on MS media supplemented with 1.0 mg/l thidiazuron (TDZ) and 0.1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The available evidences proved S-SAP marker had high level of polymorphism in strawberry accessions. (C) 2012 Elsevier B.V. All rights reserved.

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