Cloning and Characterization of New Splice Variants of Insulin-Like Growth Factor-1 Gene in Songliao Black Pig

文献类型： 外文期刊

第一作者： Liu, Chang

作者： Liu, Chang;Zhaoa, Zhihui;Bai, Wenlin;Sun, Boxing;Zhang, Yonghong;Yang, Runjun;Liu, Chang;Bai, Wenlin

作者机构：

关键词： Insulin-Like Growth Factor 1 (IGF-1);alternative splicing;Songliao Black pig;tissues;expression

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2012 年 11 卷 17 期

页码：

收录情况： SCI

摘要： Insulin-Like Growth Factor 1 (IGF-1) is a single copy gene. It generates several IGF-1 mRNA variants due to alternative splicing. Spliced variants of IGF-1 being some small peptides derived from spliced variants of IGF-1 have critical roles in cell metabolism and growth of various vertebrate species. Porcine IGF-1 gene has two promoters and the existence of Exon 6 has been confirmed in Songliao Black pig in the laboratory. The aims of the present study were to identify new spliced variants of IGF-1 gene including Exon 5 and to investigate their expression profile in different tissues of Songliao Black pig. The cDNAs of two new spliced variants of IGF-1 gene were cloned by RACE. These two variants were named Class I IGF-1-Eb and Class II IGF-1-Eb, respectively. The Class I IGF-1-Eb contains an Open Reading Frame (ORE) of 564 bp encoding 187 amino acids (GenBank Accession No. FJ91 44971. The Class II IGF-1-Eb contains an ORE of 516-bp encodnig. 171 amino acids (GenBank Accession No. FJ91 4498). Tissue distributions of mRNAs of Class I IGF-1-Eb and Class II IGF-1-Eb were investigated in Songliao Black pig by semi-quantitative RT-PCR. Both of the new mRNA variants were expressed in a variety of tissues. Class I IGF-1-Eb mRNA was more abundant than Class II IGF-1-Eb mRNA in most tissues except for duodenum and longissimus muscle (p<0.05).

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