Cloning of Acyl-ACP Thioesterase FatA from Arachis hypogaea L. and Its Expression in Escherichia coli

文献类型： 外文期刊

第一作者： Chen, Gao

作者： Chen, Gao;Peng, Zhen-ying;Shan, Lei;Xuan, Ning;Tang, Gui-ying;Zhang, Yan;Li, Lan;He, Qing-fang;Bi, Yu-ping;Chen, Gao;Peng, Zhen-ying;Shan, Lei;Xuan, Ning;Tang, Gui-ying;Zhang, Yan;Li, Lan;He, Qing-fang;Bi, Yu-ping;He, Qing-fang;Chen, Gao;Li, Lan;Bi, Yu-ping

作者机构：

期刊名称：JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY （ 影响因子：3.169； 五年影响因子：3.132 ）

ISSN： 1110-7243

年卷期： 2012 年

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收录情况： SCI

摘要： In this study, a full-length cDNA of the acyl-ACP thioesterase, AhFatA, was cloned from developing seeds of Arachis hypogaea L. by 3'-RACE. Sequence analysis showed that the open reading frame encodes a peptide of 372 amino acids and has 50-70% identity with FatA from other plants. Real-time quantitative PCR analysis revealed that AhFatA was expressed in all tissues of A. hypogaea L., but most strongly in the immature seeds harvested at 60 days after pegging. Heterologous expression of AhFatA in Escherichia coli affected bacterial growth and changed the fatty acid profiles of the membrane lipid, resulting in directed accumulation towards palmitoleic acid and oleic acid. These results indicate that AhFatA is at least partially responsible for determining the high palmitoleic acid and oleic acid composition of E. coli.

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