Isolation of Arachis hypogaea Na+/H+ antiporter and its expression analysis under salt stress

文献类型： 外文期刊

第一作者： Wan, Shubo

作者： Wan, Shubo;Meng, Jingjing;Guo, Feng;Li, Xinguo;Wan, Shubo;Meng, Jingjing;Guo, Feng;Li, Xinguo;Xing, Jinyi;Wang, Baozhi;Jia, Kunhang;Wan, Shubo;Meng, Jingjing;Guo, Feng;Li, Xinguo

作者机构：

关键词： Arachis hypogaea;AhNHX1;salt tolerance;gene expression patterns

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 65 期

页码：

收录情况： SCI

摘要： The plant Na+/H+ antiporter gene plays a major role in salt tolerance. One Na+/H+ antiporter (AhNHX1) gene was isolated from peanut (Arachis hypogaea) in the present work. The full-length cDNA of AhNHX1 was 2,331 bp, which contains a complete ORF of 1,620 bp. The deduced protein sequence contains 546 amino acids with a molecular mass of 58.8 KDa and a pI of 7.23. The amino acid sequence of the AhNHX1 shares high similarity (shows more than 81.89% identity) with those of the previously isolated NHX1 from other plants. Meanwhile, the AhNHX1 protein has twelve putative transmembrane domains, and the conserved amiloride-binding sites ((LFFIYLLPPI93)-L-84) were found in its N-terminal. Also, the expression of AhNHX1 was tissue-specific under different level NaCl treatments. Under 50 and 100 mM NaCl treatments, its expression showed higher in roots and lower in stems and leaves relative to control plants, respectively. In addition, its expression was time-specific, such that under 150 mM NaCl treatment, its expression usually increased and reached to a stable level in roots and leaves after 24 h but in stems after 48 h, respectively. These results implied that the AhNHX1 plays an important role under salt stress in peanut.

分类号：

  • 相关文献

[1]A genome-wide analysis of the lysophosphatidate acyltransferase (LPAAT) gene family in cotton: organization, expression, sequence variation, and association with seed oil content and fiber quality. Wang, Nuohan,Yu, Jiwen,Yu, Shuxun,Zhang, Jinfa,Ma, Jianjiang,Pei, Wenfeng,Wu, Man,Li, Haijing,Li, Xingli,Yu, Shuxun,Yu, Jiwen,Wang, Nuohan,Zhang, Jinfa. 2017

[2]Identification of Expressed Resistance Gene Analogs from Peanut (Arachis hypogaea L.) Expressed Sequence Tags. Liu, Zhanji,Feng, Suping,Pandey, Manish K.,Chen, Xiaoping,Culbreath, Albert K.,Liu, Zhanji,Feng, Suping,Pandey, Manish K.,Varshney, Rajeev K.,Chen, Xiaoping,Guo, Baozhu. 2013

[3]Comparative transcriptome analysis of basal and zygote-located tip regions of peanut ovaries provides insight into the mechanism of light regulation in peanut embryo and pod development. Zhang, Ye,Wang, Pengfei,Xia, Han,Zhao, Chuanzhi,Hou, Lei,Li, Changsheng,Zhao, Shuzhen,Wang, Xingjun,Zhang, Ye,Gao, Chao,Wang, Xingjun. 2016

[4]Isolation and Characterization of Putative Acetyl-CoA Carboxylases in Arachis hypogaea L.. Li, Meng-Jun,Xia, Han,Zhao, Chuan-Zhi,Li, Ai-Qin,Li, Chang-Sheng,Bi, Yu-Ping,Wan, Shu-Bo,Wang, Xing-Jun. 2010

[5]Development and application of KASP marker for high throughput detection of AhFAD2 mutation in peanut. Zhao, Shuzhen,Li, Aiqin,Li, Changsheng,Xia, Han,Zhao, Chuanzhi,Zhang, Ye,Hou, Lei,Wang, Xingjun. 2017

[6]Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum. Ding, Yu Fei,Wang, Chuan Tang,Tang, Yue Yi,Wang, Xiu Zhen,Wu, Qi,Yu, Hong Tao,Zhang, Jian Cheng,Cui, Feng Gao,Song, Guo Sheng,Yu, Shan Lin,Hu, Dong Qing,Gao, Hua Yuan. 2012

[7]The effect of low water content on seed longevity. Hu, CL,Zhang, YL,Tao, M,Hu, XR,Jiang, CY. 1998

[8]Overexpression of a novel peanut NBS-LRR gene AhRRS5 enhances disease resistance to Ralstonia solanacearum in tobacco. Chen, Hua,Cai, Tiecheng,Deng, Ye,Zhuang, Weijian,Zhang, Chong,Chen, Hua,Cai, Tiecheng,Deng, Ye,Zhuang, Ruirong,Zhang, Ning,Zeng, Yuanhuan,Zheng, Yixiong,Zhuang, Weijian,Zheng, Yixiong,Tang, Ronghua,Pan, Ronglong,Pan, Ronglong. 2017

[9]Phenotypic evaluation of the Chinese mini-mini core collection of peanut (Arachis hypogaea L.) and assessment for resistance to bacterial wilt disease caused by Ralstonia solanacearum. Jiang, Huifang,Ren, Xiaoping,Chen, Yuning,Huang, Li,Zhou, Xiaojing,Huang, Jiaquan,Liao, Boshou,Froenicke, Lutz,Yu, Jiujiang,Guo, Baozhu. 2013

[10]Characterization of a New Strain of Capsicum chlorosis virus from Peanut (Arachis hypogaea L.) in China. Chen, K.,Xu, Z.,Yan, L.,Wang, G..

[11]Applications of xerophytophysiology in plant production - peanut cultivation with the AnM method. Xu, Hui-lian,Xu, Rongyan,Qin, Feifei,Morita, Shigenori,Wang, Jingshan,Wang, Minglun,Qin, Feifei. 2009

[12]Identification and expression analysis of genes responsive to drought stress in peanut. Hou, L.,Liu, W.,Li, Z.,Huang, C.,Fang, X. L.,Wang, Q.,Liu, X.,Hou, L.,Liu, W.,Li, Z.,Huang, C.,Fang, X. L.,Wang, Q.,Liu, X.,Fang, X. L..

[13]Overexpression of a peanut NAC gene, AhNAC4, confers enhanced drought tolerance in tobacco. Tang, G. Y.,Xu, P. L.,Shan, L.,Shao, F. X.,Liu, Z. J..

[14]An efficient method for total RNA extraction from peanut seeds. Huang, C.,Picimbon, J. F.,Li, H. Q.,Li, Z.,Liu, Q.,Liu, W.,Huang, C.,Picimbon, J. F.,Li, H. Q.,Li, Z.,Liu, Q.,Liu, W..

[15]Discovery of a new mechanism for regulation of plant triacylglycerol metabolism: The peanut diacylglycerol acyltransferase-1 gene family transcriptome is highly enriched in alternative splicing variants. Zheng, Ling,Guo, Feng,Shi, Lingmin,Li, Xinguo,Shan, Lei,Wan, Shubo,Peng, Zhenying,Shockey, Jay.

[16]Cloning of metallothionein genes from Arachis hypogaea and characterization of AhMT2a. Quan, X. Q.,Shan, L.,Bi, Y. P..

[17]Comparative transcript profiling of resistant and susceptible peanut post-harvest seeds in response to aflatoxin production by Aspergillus flavus. Wang, Houmiao,Lei, Yong,Wan, Liyun,Yan, Liying,Lv, Jianwei,Ren, Xiaoping,Jiang, Huifang,Liao, Boshou,Wang, Houmiao,Lei, Yong,Wan, Liyun,Yan, Liying,Lv, Jianwei,Ren, Xiaoping,Jiang, Huifang,Liao, Boshou,Dai, Xiaofeng,Guo, Wei. 2016

[18]Identification and characterization of a gene encoding a putative lysophosphatidyl acyltransferase from Arachis hypogaea. Chen, Si-Long,Huang, Jia-Quan,Lei, Yong,Zhang, Yue-Ting,Ren, Xiao-Ping,Chen, Yu-Ning,Jiang, Hui-Fang,Yan, Li-Ying,Liao, Bo-Shou,Chen, Si-Long,Li, Yu-Rong,Chen, Si-Long.

[19]Mining Tissue-specific Contigs from Peanut (Arachis hypogaea L.) for Promoter Cloning by Deep Transcriptome Sequencing. Geng, Lili,Duan, Xiaohong,Shu, Changlong,Song, Fuping,Zhang, Jie,Liang, Chun,Liang, Chun.

[20]Rhizobium pakistanensis sp nov., isolated from groundnut (Arachis hypogaea) nodules grown in rainfed Pothwar, Pakistan. Khalid, Rabia,Ali, Safdar,Amara, Ummay,Hayat, Rifat,Khalid, Rabia,Zhang, Yu Jing,Sui, Xin Hua,Chen, Wen Xin,Khalid, Rabia,Zhang, Yu Jing,Sui, Xin Hua,Chen, Wen Xin,Zhang, Xiao Xia.

作者其他论文 更多>>

微信小程序