Function identification of bovine Nramp1 promoter and intron 1

文献类型： 外文期刊

第一作者： Hao, Linlin

作者： Hao, Linlin;Zhang, Libo;Liu, Songcai;Li, Mingtang;Zhong, Jifeng;Wang, Nan

作者机构：

关键词： bovine;Nrampl;5'-flanking region;promoter

期刊名称：ANIMAL CELLS AND SYSTEMS （ 影响因子：1.815； 五年影响因子：1.336 ）

ISSN： 1976-8354

年卷期： 2011 年 15 卷 3 期

页码：

收录情况： SCI

摘要： The Nrampl/Slc1 la1 locus encodes a proton-coupled divalent cation transporter, expressed in late endosomes/lysosomes of macrophages, that constitutes a component of the innate immune response to combat intracellular pathogens and it was shown to play an important role in regulating inherent immunity. The previously identified Z-DNA forming polymorphic repeat(GT)n in the promoter region of the human Nrampl gene does act as a functional polymorphism influencing gene expression. Research has shown that INF-gamma, TNF-alpha, IL-1 beta and bacteria LPS increase the level of Nrampl expression. However, the molecular mechanism for Nrampl gene regulation is unclear. In this research, bovine Nrampl 5'-flanking region (-1748 similar to +769) was cloned and analyzed by bioinformatics. Then to find the core promoter and the cis-acting elements, deletion analysis of promoter was performed using a set of luciferase reporter gene constructs containing successive deletions of the bovine Nrampl 5'-flanking regions. Promoter activity analysis by the dual luciferase reporter assay system showed that the core promoter of Nrampl was located at +58 similar to -89 bp. Some positive regulatory elements are located at -89 similar to -205 bp and -278 similar to -1495 bp. And the repressor elements were in region -205 similar to -278 bp, intronl and -1495 similar to -1748 bp. LPS-responsive regions were located at -1495 similar to -1748 bp and -278 similar to -205 bp. The present study provides an initial effort to explore the molecular mechanism of transcriptional activation of the bovine Nrampl gene and should facilitate further studies to decode the complex regulatory process and for molecular breeding for disease resistance in bovines.

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