Genetic analysis of wild apple resources in Shandong province based on inter-simple sequence repeats (ISSR) and sequence-specific amplification polymorphism (S-SAP) markers
文献类型: 外文期刊
第一作者: He, Ping
作者: He, Ping;Li, Linguang;Li, Huifeng;Wang, Haibo;Yang, Jianming;Wang, Yuxia
作者机构:
关键词: Apple;wild resource;inter-simple sequence repeats (ISSR);sequence-specific amplification polymorphism (S-SAP)
期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )
ISSN: 1684-5315
年卷期: 2011 年 10 卷 46 期
页码:
收录情况: SCI
摘要: Apple (malus domestica Borkh.), which is a widely cultivated, important and economic fruit crop with nutritive and medicinal importance, has emerged as a model horticultural crop in this post-genomic era. Wild apple resources are important and they develop gradually in apple industry and genetic diversity. In this study, two molecular markers (inter-simple sequence repeats, ISSR and sequence-specific amplification polymorphism, S-SAP) were evaluated for genetic analysis of 31 wild apple resources in Shandong. A total of 20 ISSR primers were used and 110 polymorphic bands were amplified. Five S-SAP primer sets yielded a total of 496 bands, of which 201 (40.52%) were polymorphic. The similarity coefficient between resources ranged from 0.70 to 0.94 for ISSR analysis and from 0.66 to 0.95 using the S-SAP methodology. This study indicates that the results obtained based on the dendrograms constructed using unweighted pair-group using arithmetic average (UPGMA) cluster analysis were significantly correlated. The ISSR and S-SAP markers were found to be useful for wild apple resources identification and assessment of phonetic relationships.
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