Preparation of an anti-diethylstilbestrol monoclonal antibody and development of an indirect competitive ELISA to detect diethylstilbestrol in biological samples
文献类型: 外文期刊
第一作者: Li WeiHua
作者: Li WeiHua;Liu Wei;Yin WeiWei;Xu Jing;Liu JinTing;Meng Meng;Xue HuYin;Xi RiMo;Meng Meng;Xue HuYin;Xi RiMo;He FangYang;Wan YuPing;Feng CaiWei;Wang ShanLiang;Lu Xiao
作者机构:
关键词: diethylstilbestrol;ELISA;monoclonal antibody;drug residue
期刊名称:CHINESE SCIENCE BULLETIN ( 影响因子:1.649; 五年影响因子:1.738 )
ISSN: 1001-6538
年卷期: 2011 年 56 卷 8 期
页码:
收录情况: SCI
摘要: Based on the preparation of an anti-diethylstilbestrol (DES) monoclonal antibody, a simple and convenient indirect competitive enzyme-linked immunosorbent assay (ELISA) method for DES detection has been developed. The monoclonal antibody demonstrated high sensitivity to DES with an IC(50) value of 275 pg mL(-1) and detection limit (LOD) of 90 pg mL(-1). The specificity of the assay was studied by measuring cross-reactivity of the antibody with structurally related compounds of ethinyl estradiol (< 7%), estrone (< 0.1%), estriol (< 0.1%), and diethylstilbestrol benzoate (< 0.1%). Chicken, fish, shrimp, urine and bile spiked with different concentration of DES were detected by the developed method, and the recovery rates were greater than 79.5%. Intra- and inter-assay variations were about 6%. This method exhibited high stability with a coefficient of variation less than 10% in buffer and in real samples. The LODs in fish/shrimp, liver, feed and urine spiked with DES were 600, 600, 4800 and 600 pg mL(-1), respectively. These results confirmed that the antibody to DES was successfully produced and could be used to establish ELISA methods for DES detection in food producing animals.
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