Study on the Hemolysin Phenotype and the Genetype Distribution of Staphyloccocus aureus Caused Bovine Mastitis in Shandong Dairy Farms

文献类型： 外文期刊

第一作者： Wang Fei

作者： Wang Fei;Yang Hongjun;He Hong-bin;Wang Changfa;Gao Yundong;Zhong Qifeng;Wang Fei;Wang Xiaohong;Zeng Yanjun

作者机构：

关键词： Staphylococcus aureus;Mastitis;Hemolysin phenotype;genetype distribution

期刊名称：INTERNATIONAL JOURNAL OF APPLIED RESEARCH IN VETERINARY MEDICINE （ 影响因子：0.306； 五年影响因子：0.339 ）

ISSN： 1542-2666

年卷期： 2011 年 9 卷 4 期

页码：

收录情况： SCI

摘要： Object: To develop a typing method for Staphylococcal hemolysin genes, and to research on the distribution of hemolysin phenotype and hemolysin genes in Staphylococcus aureus isolates obtained from milk of cow suffered mastitis and to analysis there relevance. Methods: The hemolysin phenotype was oberserved with the method of 5% blood plate. Hemolysin genes were assessed via polymerase chain reaction. Results: The typing method for Staphylococccal hemolysin phenotype and genes with PCR was developed. The isolates with alpha- hemolysis are 56 in 129 isolates, account for 43.4%; 43 show beta-hemolysis, account for 34.11%; Another 29 isolates showed no hemolysis phenotype, and it account for 22.48%. The isolates with hla gene account for 34.88%, hlb gene account for 42.60%. Conclusion: In the hemolytic phenotype, alpha- hemolysis are more than beta-hemolysis, however the hlb gene detection rate is higher than the hla gene. The distribution of hemolysin phenotype and hemolysin genes is not a corresponding relationship of Staphylococcus aureus isolates from raw milk of cow. The study can provide a basis for prevention and treatment of mastitis in dairy cows caused by Staphylococcus aureus.

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