Specific Expression of Maize SBEIIb Promoter Mediated by Different Promoter Region in Transgenic Tobacco Plants

文献类型： 外文期刊

第一作者： Sun Cui-xia

作者： Sun Cui-xia;Li Meng;Wang Xiao-peng;Zhang Guo-dong;Tian Yan-chen;Wang Ze-li;Han Jing

作者机构：

关键词： maize starch-branching enzyme;promoter;cloning;specific expression

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2009 年 8 卷 11 期

页码：

收录情况： SCI

摘要： Starch branching enzyme (SBE) catalyzes the biosynthesis of amylopectin. We described the isolation and characterization of SBEIIb promoter and their expression patterns in transgenic tobacco. Using the genomic DNA of maize cultivar Lunuo 1 as template, the SBEIIb promoter was isolated by PCR and was cloned into pMD18-T vector. To study SEBIIb gene regulation at the cellular level, SBEIIb promoter was fused to the beta-glucuronidase (GUS) report gene. The results of the fluorometric GUS assays indicate that the sbeIIb-GUS fusion directed a seed-specific expression. Four series of constructs were made with the promoter and the GUS reporter gene to investigate the cis-acting analysis, showing that the four different constructs all can drive expression of the GUS gene in seed plumule and cotyledon and the GUS activity was apparently decreased with the progressive loss of promoter 5' end.

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