Polyclonal antibodies to grain gliadins and their application in wheat quality prediction

文献类型： 外文期刊

第一作者： Li, Q. Y.

作者： Li, Q. Y.;Song, X. Y.;Zhang, E. Y.;Pei, Y. H.;Li, Q. Y.;Ji, K. M.;Yan, Y. M.

作者机构：

关键词： polyclonal antibodies;ELISA;gliadins;wheat quality

期刊名称：CEREAL RESEARCH COMMUNICATIONS （ 影响因子：0.85； 五年影响因子：1.067 ）

ISSN： 0133-3720

年卷期： 2008 年 36 卷 1 期

页码：

收录情况： SCI

摘要： Gliadins were extracted from three wheat varieties, viz. Nongda 99260037 (good quality), Henan 9023 (media quality) and Nongda 98123 (poor quality), and alpha-, beta-, gamma-, omega-gliadins were purified from Nongda 99260037 using preparative SDS-PAGE. The total gliadins and alpha-, beta-, gamma-, omega-gliadins were used as antigens to immunise BALB/C mice, and the corresponding polyclonal antibodies were prepared, designated as Anti-A, Anti-B, Anti-C, Anti-A alpha, Anti-A beta, Anti-A gamma and Anti-A omega, respectively. Binding of the polyclonal antibodies with 8 varieties, that varied in quality properties, showed correlations with some wheat quality parameters. Correlation coefficients between antibodies against total gliadins or gamma-, and omega-gliadin of Nongda 99260037 and quality parameters were higher than other antibodies. Of seven polyclonal antibodies tested, three (Anti-A gamma, Anti-A omega and Anti-A) displayed significant positive or negative associations between antibody binding and dough development time, strength, valorimenter value and stability time, but no significant correlations were observed with water absorption. These results suggest that polyclonal antibodies could be used for rapid prediction and screening of wheat quality parameters.

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